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pubmed-article:1515116pubmed:abstractTextIt is shown that a streptavidin monolayer immobilized onto an evaporated gold film with biotin forms the basis of a highly specific sensing element. As an example, we show that by immobilizing the biotinylated antibody sex hormone binding globulin (alpha-SHBG) to the bound streptavidin monolayer a specific sensor for the antigen SHBG is readily fabricated. The interaction between immobilized antibody and corresponding antigen is monitored by surface plasmon resonance spectroscopy and is shown to follow a classic Langmuir isotherm. Detection of SHBG at nanomolar concentrations is demonstrated.lld:pubmed
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pubmed-article:1515116pubmed:articleTitleA surface plasmon resonance immunosensor based on the streptavidin-biotin complex.lld:pubmed
pubmed-article:1515116pubmed:affiliationInstitute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.lld:pubmed
pubmed-article:1515116pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:1515116pubmed:publicationTypeResearch Support, Non-U.S. Gov'tlld:pubmed
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