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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1992-10-2
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pubmed:abstractText |
It is shown that a streptavidin monolayer immobilized onto an evaporated gold film with biotin forms the basis of a highly specific sensing element. As an example, we show that by immobilizing the biotinylated antibody sex hormone binding globulin (alpha-SHBG) to the bound streptavidin monolayer a specific sensor for the antigen SHBG is readily fabricated. The interaction between immobilized antibody and corresponding antigen is monitored by surface plasmon resonance spectroscopy and is shown to follow a classic Langmuir isotherm. Detection of SHBG at nanomolar concentrations is demonstrated.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0956-5663
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
7
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
405-10
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pubmed:dateRevised |
2009-7-14
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pubmed:meshHeading |
pubmed-meshheading:1515116-Antigens,
pubmed-meshheading:1515116-Bacterial Proteins,
pubmed-meshheading:1515116-Biosensing Techniques,
pubmed-meshheading:1515116-Biotechnology,
pubmed-meshheading:1515116-Biotin,
pubmed-meshheading:1515116-Evaluation Studies as Topic,
pubmed-meshheading:1515116-Humans,
pubmed-meshheading:1515116-Immunoassay,
pubmed-meshheading:1515116-Sex Hormone-Binding Globulin,
pubmed-meshheading:1515116-Spectrum Analysis,
pubmed-meshheading:1515116-Streptavidin
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pubmed:year |
1992
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pubmed:articleTitle |
A surface plasmon resonance immunosensor based on the streptavidin-biotin complex.
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pubmed:affiliation |
Institute of Molecular and Biomolecular Electronics, University of Wales, Bangor, Gwynedd, UK.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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