Linked Life Data

15149609

Source:http://linkedlifedata.com/resource/pubmed/id/15149609

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2004-5-19
pubmed:abstractText
An improved technique for the analysis of phosphatidylcholine (PC) monohydroperoxides was developed using quadrupole time-of-flight (Q-TOF) mass spectrometry with electrospray ionization. Separation was obtained using an HPLC C8 column with a gradient of methanol and 10 mM aqueous ammonium acetate. Monohydroperoxides of palmitoyl-linoleoyl (C16:0/C18:2) PC, stearoyl-linoleoyl (C18:0/C18:2) PC, and oleoyl-linoleoyl (C18:1/C18:2) PC were detected mainly as MH(+) and [M+Na](+) ions in the heart of the intact rat. Using standard synthetic PCOOH (C16:0/C18:2-OOH), the lipid extract component was identified as (C16:0/C18:2-OOH) PC based on the product ions of ESI-MS-MS and, the PCOOH concentration was quantitated using HPLC with chemiluminescence detection. Two epoxyhydroxy derivatives of the three PCs mentioned above were also detected. This is the first report to show the presence of monohydroperoxides and epoxyhydroxy-derivatives of (C16:0/C18:2)PC, (C18:0/C18:2)PC, and (C18:1/C18:2) PC in the rat heart.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1570-0232
pubmed:author
pubmed:issnType
Print
pubmed:day
25
pubmed:volume
806
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Determination of phosphatidylcholine monohydroperoxides using quadrupole time-of-flight mass spectrometry.
pubmed:affiliation
Department of Legal Medicine, Kobe University Graduate School of Medicine, 7 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan. adachi@med.kobe-u.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't