rdf:type |
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lifeskim:mentions |
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pubmed:issue |
8
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pubmed:dateCreated |
1992-9-29
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pubmed:abstractText |
A collection of 521 environmental isolates of Vibrio cholerae which were previously examined by the suckling mouse assay and found to be negative for the heat-stable enterotoxin NAG-ST were reassessed by a recently developed DNA probe for NAG-ST. A total of 12 (2.3%) of the isolates hybridized with the NAG-ST probe. By using a cholera toxin (CT) DNA probe, the CT gene was detected in six of the strains in the collection, although none of the isolates of V. cholerae non-O1 hybridized with both of the toxin probes. All of the NAG-ST and CT probe-positive strains were hemolysin positive. Thirty-fold-concentrated supernatants of the three representative NAG-ST DNA probe-positive V. cholerae non-O1 strains gave positive fluid accumulation ratios in the suckling mouse assay even after heating (100 degrees C for 5 min) and also inhibited the binding of a NAG-ST monoclonal antibody to the bound NAG-ST in a competitive enzyme-linked immunosorbent assay (ELISA). Likewise, all six CT probe-positive V. cholerae non-O1 strains produced in vitro CT when examined by the CT bead ELISA. HindIII digest patterns of chromosomal DNA from the representative NAG-ST gene-positive strains were visually indistinguishable. Between the groups of NAG-ST probe-positive strains examined, there was a variation in the hybridizable fragments, with one group of strains exhibiting a hybridizable fragment similar to that of the NRT 36 reference strain; a smaller HindIII fragment hybridized with the NAG-ST probe in the other group of strains.(ABSTRACT TRUNCATED AT 250 WORDS)
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-1195397,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-14292746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-1584072,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-1855646,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-2312721,
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0099-2240
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:volume |
58
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2485-9
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pubmed:dateRevised |
2010-9-7
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pubmed:meshHeading |
pubmed-meshheading:1514795-Animals,
pubmed-meshheading:1514795-Animals, Suckling,
pubmed-meshheading:1514795-Biological Assay,
pubmed-meshheading:1514795-DNA Probes,
pubmed-meshheading:1514795-Enterotoxins,
pubmed-meshheading:1514795-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:1514795-Genes, Bacterial,
pubmed-meshheading:1514795-India,
pubmed-meshheading:1514795-Mice,
pubmed-meshheading:1514795-Serotyping,
pubmed-meshheading:1514795-Vibrio cholerae,
pubmed-meshheading:1514795-Water Microbiology
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pubmed:year |
1992
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pubmed:articleTitle |
Reassessment of the prevalence of heat-stable enterotoxin (NAG-ST) among environmental Vibrio cholerae non-O1 strains isolated from Calcutta, India, by using a NAG-ST DNA probe.
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pubmed:affiliation |
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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