Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1992-9-29
pubmed:abstractText
A collection of 521 environmental isolates of Vibrio cholerae which were previously examined by the suckling mouse assay and found to be negative for the heat-stable enterotoxin NAG-ST were reassessed by a recently developed DNA probe for NAG-ST. A total of 12 (2.3%) of the isolates hybridized with the NAG-ST probe. By using a cholera toxin (CT) DNA probe, the CT gene was detected in six of the strains in the collection, although none of the isolates of V. cholerae non-O1 hybridized with both of the toxin probes. All of the NAG-ST and CT probe-positive strains were hemolysin positive. Thirty-fold-concentrated supernatants of the three representative NAG-ST DNA probe-positive V. cholerae non-O1 strains gave positive fluid accumulation ratios in the suckling mouse assay even after heating (100 degrees C for 5 min) and also inhibited the binding of a NAG-ST monoclonal antibody to the bound NAG-ST in a competitive enzyme-linked immunosorbent assay (ELISA). Likewise, all six CT probe-positive V. cholerae non-O1 strains produced in vitro CT when examined by the CT bead ELISA. HindIII digest patterns of chromosomal DNA from the representative NAG-ST gene-positive strains were visually indistinguishable. Between the groups of NAG-ST probe-positive strains examined, there was a variation in the hybridizable fragments, with one group of strains exhibiting a hybridizable fragment similar to that of the NRT 36 reference strain; a smaller HindIII fragment hybridized with the NAG-ST probe in the other group of strains.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-14292746, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-1584072, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-1696928, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-1855646, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-2205577, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-2312721, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-2380369, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-2584765, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-2862364, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-3055182, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-3143897, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-3223774, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-3305559, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-3570456, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-367273, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-3779031, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-387599, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-3890578, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-3957432, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-4891264, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-4894736, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-5640984, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-599729, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-6140426, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-6341083, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-6411622, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-6625378, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514795-7298194
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2485-9
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Reassessment of the prevalence of heat-stable enterotoxin (NAG-ST) among environmental Vibrio cholerae non-O1 strains isolated from Calcutta, India, by using a NAG-ST DNA probe.
pubmed:affiliation
National Institute of Cholera and Enteric Diseases, Beliaghata, Calcutta, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't