Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1992-9-29
pubmed:databankReference
pubmed:abstractText
Phage phi 197 is representative of a widespread lactococcal phage group characterized by a particular morphology (prolate head with a noncontractile tail). In order to develop an immunoenzymatic phage detection test, fusion proteins containing beta-galactosidase fused to epitopes of phage phi 197 structural proteins were constructed by cloning random DNA fragments from the phage genome upstream of a lacZ gene on a plasmid vector. Recombinant plasmids containing certain fragments encoded the synthesis of fusion proteins which react with polyclonal antibodies against the phage and confer a Lac+ phenotype on Escherichia coli. Three different epitopes were represented; phage-specific DNA fragments encoding these epitopes were mapped at three locations on the phage genome, and their nucleotide sequences were determined. Two fused phage antigens were conformational epitopes, whereas the phage epitope of protein encoded by the recombinant plasmid designated pOA17 was a denaturation-resistant epitope. This epitope was very immunogenic. Protein encoded by plasmid pOA17 was synthesized in large amounts from a strong promoter. Antibodies raised against this hybrid protein were used to identify the 46-kDa minor phage protein which provides the epitope. Antibody cross-reactivity of phages related to phi 197 showed that this epitope is well conserved in this genetic group.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-16347374, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-16350018, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-1901837, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-2167627, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-271968, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-2826650, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-2844302, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-2987525, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-2999317, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-3016319, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-3116882, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-3128635, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-3139060, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-3507689, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-6161559, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-6308625, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-6324674, http://linkedlifedata.com/resource/pubmed/commentcorrection/1514794-6436145
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:geneSymbol
lacZ
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2479-84
pubmed:dateRevised
2010-9-7
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Characterization of Lactococcus lactis phage antigens.
pubmed:affiliation
Centre de Recherche de Biochimie et de Génétique Cellulaires, Centre National de la Recherche Scientifique, Toulouse, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't