Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2004-7-26
pubmed:abstractText
Clathrin-independent endocytosis internalizes plasma membrane proteins that lack cytoplasmic sequences recognized by clathrin adaptor proteins. There is evidence for different clathrin-independent pathways but whether they share common features has not been systematically tested. Here, we examined whether CD59, an endogenous glycosylphosphatidyl inositol-anchored protein (GPI-AP), and major histocompatibility protein class I (MHCI), an endogenous, integral membrane protein, entered cells through a common mechanism and followed a similar itinerary. At early times of internalization, CD59 and MHCI were found in the same Arf6-associated endosomes before joining clathrin cargo proteins such as transferrin in common sorting endosomes. CD59 and MHCI, but not transferrin, also were observed in the Arf6-associated tubular recycling membranes. Endocytosis of CD59 and MHCI required free membrane cholesterol because it was inhibited by filipin binding to the cell surface. Expression of active Arf6 stimulated endocytosis of GPI-APs and MHCI to the same extent and led to their accumulation in Arf6 endosomes that labeled intensely with filipin. This blocked delivery of GPI-APs and MHCI to early sorting endosomes and to lysosomes for degradation. Endocytosis of transferrin was not affected by any of these treatments. These observations suggest common mechanisms for endocytosis without clathrin.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-10198050, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-10547351, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-10589680, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-10930469, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11071915, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11148137, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11257904, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11273133, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11331304, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11331875, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11454454, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11463390, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11535619, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11549256, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11682487, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11707525, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11890541, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-11970892, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12032069, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12047552, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12082155, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12142266, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12145200, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12221119, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12383800, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12447393, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12482757, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12543707, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12589044, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12621426, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12657640, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12771123, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-12925711, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-13679513, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-14612561, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-14617356, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-14675422, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-14734557, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-8505326, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-9314528, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-9531551, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-9658170, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-9664047, http://linkedlifedata.com/resource/pubmed/commentcorrection/15146059-9707422
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1059-1524
pubmed:author
pubmed:issnType
Print
pubmed:volume
15
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3542-52
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Characterization of a nonclathrin endocytic pathway: membrane cargo and lipid requirements.
pubmed:affiliation
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, Maryland 20892, USA.
pubmed:publicationType
Journal Article