Linked Life Data

15142604

Source:http://linkedlifedata.com/resource/pubmed/id/15142604

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2004-5-14
pubmed:abstractText
A magnetoelastic bioaffinity sensor coupled with biocatalytic precipitation is described for avidin detection. The non-specific adsorption characteristics of streptavidin on different functionalized sensor surfaces are examined. It is found that a biotinylated poly(ethylene glycol) (PEG) interface can effectively block non-specific adsorption of proteins. Coupled with the PEG immobilized sensor surface, alkaline phosphatase (AP) labeled streptavidin is used to track specific binding on the sensor. This mass-change-based signal is amplified by the accumulation on the sensor of insoluble products of 5-bromo-4-chloro-3-indolyl phosphate catalyzed by AP. The resulting mass loading on the sensor surface in turn shifts the resonance frequency of the magnetoelastic sensors, with an avidin detection limit of approximately 200 ng/ml.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0956-5663
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1695-701
pubmed:dateRevised
2009-7-14
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
A magnetoelastic bioaffinity-based sensor for avidin.
pubmed:affiliation
Department of Electrical Engineering, 217 Materials Research Laboratory, The Pennsylvania State University, University Park, Pennsylvania, PA 16802, USA.
pubmed:publicationType
Journal Article, Comparative Study, Evaluation Studies, Validation Studies