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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
2004-5-13
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pubmed:abstractText |
The immunogenicity of HLA-A2-restricted T-cell epitopes in the S protein of the Severe acute respiratory syndrome coronavirus (SARS-CoV) and of human coronavirus strain 229e (HCoV-229e) was analyzed for the elicitation of a T-cell immune response in donors who had fully recovered from SARS-CoV infection. We employed online database analysis to compare the differences in the amino acid sequences of the homologous T epitopes of HCoV-229e and SARS-CoV. The identified T-cell epitope peptides were synthesized, and their binding affinities for HLA-A2 were validated and compared in the T2 cell system. The immunogenicity of all these peptides was assessed by using T cells obtained from donors who had fully recovered from SARS-CoV infection and from healthy donors with no history of SARS-CoV infection. HLA-A2 typing by indirect immunofluorescent antibody staining showed that 51.6% of SARS-CoV-infected patients were HLA-A2 positive. Online database analysis and the T2 cell binding test disclosed that the number of HLA-A2-restricted immunogenic epitopes of the S protein of SARS-CoV was decreased or even lost in comparison with the homologous sequences of the S protein of HCoV-229e. Among the peptides used in the study, the affinity of peptides from HCoV-229e (H77 and H881) and peptides from SARS-CoV (S978 and S1203) for binding to HLA-A2 was higher than that of other sequences. The gamma interferon (IFN-gamma) release Elispot assay revealed that only SARS-CoV-specific peptides S1203 and S978 induced a high frequency of IFN-gamma-secreting T-cell response in HLA-A2(+) donors who had fully recovered from SARS-CoV infection; such a T-cell epitope-specific response was not observed in HLA-A2(+) healthy donors or in HLA-A2(-) donors who had been infected with SARS-CoV after full recovery. Thus, T-cell epitopes S1203 and S978 are immunogenic and elicit an overt specific T-cell response in HLA-A2(+) SARS-CoV-infected patients.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15140958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15140958-10602881,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15140958-10961675,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15140958-6300299,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/15140958-9658094
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-538X
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pubmed:author |
pubmed-author:ChenWei-FengWF,
pubmed-author:ChengChak-Sum SamuelCS,
pubmed-author:GuoZhi-HongZH,
pubmed-author:HaarD JDJ,
pubmed-author:HeXiao-YanXY,
pubmed-author:LaiSik-ToST,
pubmed-author:MengLiL,
pubmed-author:NgJoice Na LeeJN,
pubmed-author:PangXue-WenXW,
pubmed-author:SinWan-Yee FionWY,
pubmed-author:WangYue-DanYD,
pubmed-author:WongTin-yauTY,
pubmed-author:XieYongY,
pubmed-author:XuGuo-BingGB,
pubmed-author:YangHuang-HaoHH,
pubmed-author:YangHuang-HuaHH,
pubmed-author:YangRui-FengRF,
pubmed-author:ZhangHua-GangHG
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pubmed:issnType |
Print
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pubmed:volume |
78
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5612-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15140958-Amino Acid Sequence,
pubmed-meshheading:15140958-Epitopes, T-Lymphocyte,
pubmed-meshheading:15140958-HLA-A2 Antigen,
pubmed-meshheading:15140958-Humans,
pubmed-meshheading:15140958-Interferon-gamma,
pubmed-meshheading:15140958-Membrane Glycoproteins,
pubmed-meshheading:15140958-Molecular Sequence Data,
pubmed-meshheading:15140958-SARS Virus,
pubmed-meshheading:15140958-Severe Acute Respiratory Syndrome,
pubmed-meshheading:15140958-T-Lymphocytes,
pubmed-meshheading:15140958-Viral Envelope Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
T-cell epitopes in severe acute respiratory syndrome (SARS) coronavirus spike protein elicit a specific T-cell immune response in patients who recover from SARS.
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pubmed:affiliation |
Department of Immunology, Peking University Health Science Centre, 38, Xueyuanlu, Beijing, 100083, People's Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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