Linked Life Data

1513933

Source:http://linkedlifedata.com/resource/pubmed/id/1513933

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1992-9-30
pubmed:abstractText
Cationic lipids are shown to be efficient DNA carriers for gene transfer into neurons and chromaffin cells. Chimeric genes containing the coding sequence for the bacterial gene chloramphenicol acetyl transferase (CAT), under various promoter sequences were used to estimate transfection efficiency and to analyse transcriptional mechanisms. Using a ubiquitously expressed construct (RSV-CAT), and an anti-CAT antibody to identify CAT positive cells transfection efficiency was found to be approximately 70% in cultures of hypothalamic neurons. Proenkephalin gene expression was studied in chromaffin cells by employing a chimeric gene containing the rat pro-enkephalin promoter fused to the CAT reporter gene. Using this model we show that the transfected gene is inducible by neurotransmitters and second messengers. The contribution of a specific kinase (protein kinase A, PKA) to proenkephalin gene regulation is analysed using this model system, and expression vectors coding for the catalytic subunit of PKA.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0278-5846
pubmed:author
pubmed:issnType
Print
pubmed:volume
16
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
959-68
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Genetic analyses in neurons and neural crest-derived post mitotic cells.
pubmed:affiliation
Laboratoire de Physiologie Générale, URA 1446 CNRS, Université Louis Pasteur, Strasbourg, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't