Source:http://linkedlifedata.com/resource/pubmed/id/15138888
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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0020792,
umls-concept:C0162610,
umls-concept:C0449432,
umls-concept:C0682323,
umls-concept:C1166795,
umls-concept:C1179435,
umls-concept:C1511545,
umls-concept:C1514562,
umls-concept:C1524073,
umls-concept:C1548799,
umls-concept:C1705248,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221
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pubmed:issue |
5
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pubmed:dateCreated |
2004-6-15
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pubmed:abstractText |
Successful cell division requires proper assembly, placement and functioning of the spindle apparatus that segregates the chromosomes. The Caenorhabditis elegans gene lin-5 encodes a novel coiled-coil component of the spindle required for spindle positioning and chromosome segregation. To gain further insights into lin-5 function, we screened for dominant suppressors of the partial loss-of-function phenotype associated with the mutation lin-5(ev571ts ), and isolated 68 suppressing mutations. Eight out of the ten suppressors sequenced contained intragenic missense mutations immediately upstream of the lesion in lin-5(ev571ts ). These probably help to stabilize protein-protein interactions mediated by the coiled-coil domain. This domain was found to be required for binding to several putative LIN-5 interacting (LFI) proteins identified in yeast two-hybrid screens. Interestingly, interaction with the coiled-coil protein LFI-1 was specifically reduced by the lin-5(ev571ts ) mutation and restored by a representative intragenic suppressor mutation. Immunostaining experiments showed that LIN-5 and LFI-1 may co-localize around the kinetochore microtubules during metaphase, indicating potential interaction in vivo. The coiled-coil domain of LIN-5 was also found to mediate homodimerization, while the C-terminal region of LIN-5 was sufficient for interaction with GPR-1, a recently identified component of a LIN-5 spindle-regulatory complex. A single amino-acid substitution in the N-terminal region of LIN-5, encoded by the e1457 allele, abolished all LIN-5 interactions. Taken together, our results indicate that the spindle functions of LIN-5 depend on interactions with multiple protein partners, and that these interactions are mediated through several different domains of LIN-5.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Cell Cycle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/G protein regulator 1, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/G protein regulator 2, C elegans,
http://linkedlifedata.com/resource/pubmed/chemical/lin-5 protein, C elegans
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1617-4615
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
532-44
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15138888-Amino Acid Sequence,
pubmed-meshheading:15138888-Animals,
pubmed-meshheading:15138888-Base Sequence,
pubmed-meshheading:15138888-Caenorhabditis elegans,
pubmed-meshheading:15138888-Caenorhabditis elegans Proteins,
pubmed-meshheading:15138888-Cell Cycle Proteins,
pubmed-meshheading:15138888-Genes, Helminth,
pubmed-meshheading:15138888-Mitosis,
pubmed-meshheading:15138888-Mitotic Spindle Apparatus,
pubmed-meshheading:15138888-Molecular Sequence Data,
pubmed-meshheading:15138888-Mutation,
pubmed-meshheading:15138888-Two-Hybrid System Techniques
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pubmed:year |
2004
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pubmed:articleTitle |
Identification of critical domains and putative partners for the Caenorhabditis elegans spindle component LIN-5.
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pubmed:affiliation |
Massachusetts General Hospital Cancer Center (Bldg. 149), Harvard Medical School, Charlestown, MA 02129, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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