Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-5-12
pubmed:abstractText
A 55-kDa protein named AILb-A, isolated from the seed extract of Aeginetia indica L., a parasitic plant, induces a Th1-type T-cell response and elicits a marked antitumor effect in tumor-bearing mice. In the present study, we examined the role of Toll-like receptors (TLRs), which have been implicated in pathogen-induced cell signaling, in AILb-A-induced immune responses. In the luciferase assay using a nuclear factor (NF)-kappaB-dependent reporter plasmid, AILb-A induced NF-kappaB activation in the cells transfected with TLR4, but not with those transfected with the TLR2 gene, in a dose-dependent manner. TLR4-mediated NF-kappaB activation induced by AILb-A but not by lipopolysaccharide (LPS) was also observed under serum-free conditions. In in vitro experiments using human peripheral blood mononuclear cells, AILb-A-induced cytokine production was markedly inhibited by anti-TLR4 but not by anti-CD14 antibody, while LPS-induced, TLR4-mediated cytokine production was inhibited by anti-CD14 as well as anti-TLR4 antibodies. Cytokine production, killer cell activities, maturation of dendritic cells, phosphorylation of mitogen-activated protein kinases, and nuclear translocation of interferon-regulatory factor 3 induced by AILb-A were severely impaired in TLR4-deficient but not TLR2-deficient mice. Transfection of TLR4-deficient mouse-derived macrophages with the TLR4 expression plasmid led AILb-A to induce cytokines. Finally, the antitumor effect of AILb-A was also impaired in TLR4-deficient and TLR4-mutated mice. These findings suggest that TLR4 mediates antitumor immunity induced by the plant-derived protein AILb-A.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10201887, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10359581, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10463604, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10559223, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10586073, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10588727, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10644670, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10725698, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10835634, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10854325, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-1090367, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10963608, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-10996035, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-11044375, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-11083809, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-11130078, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-11499808, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-11526399, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-11562070, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-11698465, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-11751856, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-12591988, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-1638554, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-3293775, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-367973, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-679208, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-7226139, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-8124709, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-8206751, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-8557520, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-8808632, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-915305, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-9435236, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-9665271, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-9734363, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-9851930, http://linkedlifedata.com/resource/pubmed/commentcorrection/15138173-9989976
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Monoclonal, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Ly, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/IRF3 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Immunologic Factors, http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-3, http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma, http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12, http://linkedlifedata.com/resource/pubmed/chemical/Irf3 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/LY96 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides, http://linkedlifedata.com/resource/pubmed/chemical/Ly96 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Antigen 96, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Mitogen-Activated Protein Kinases, http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B, http://linkedlifedata.com/resource/pubmed/chemical/Peptidoglycan, http://linkedlifedata.com/resource/pubmed/chemical/Plant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface, http://linkedlifedata.com/resource/pubmed/chemical/TLR2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/TLR4 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 2, http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 4, http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptors, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1071-412X
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
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