Source:http://linkedlifedata.com/resource/pubmed/id/15134341
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3-4
|
| pubmed:dateCreated |
2004-5-11
|
| pubmed:abstractText |
Adenine nucleotides act through specific cell surface receptors to invoke a variety of biological responses. Here we show that cells of neuroepithelial bodies (NEB), presumed O2 airway sensors in neonatal hamster lung, express functional P2X receptors (P2X-R). Positive immunostaining was detected in NEB cells using double-label immunohistochemistry with antibodies against P2X2 and P2X3 receptor subunits, which co-localized with serotonin (5-HT), a marker of NEB cells. For electrophysiological characterization of P2X2-R in NEB cells, fresh neonatal hamster lung slice preparation was used. Under whole-cell patch clamp, perfusion with ATP induced a concentration-dependent, non-desensitizing inward current (EC50=12 microM). Perfusion with alpha,beta-methylene ATP also induced a slow-desensitizing inward current (EC50=8.2 microM). Suramin (IC50 ca. 43 microM) and TNP-ATP (IC50 ca. 8 microM) blocked the currents evoked by both ATP and alpha,beta-methylene ATP. Using carbon fiber amperometry we observed that hypoxia and ATP induced 5-HT release from NEB cells and that this release was blocked by suramin. These data suggest that functional P2X2/3 heteromeric receptors are expressed in NEB cells. The possible function of these purinoreceptors in NEB cells could include modulation of hypoxia chemotransmission.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Purinergic Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Purinergic,
http://linkedlifedata.com/resource/pubmed/chemical/Serotonin
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1431-6730
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
385
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
275-84
|
| pubmed:dateRevised |
2010-11-18
|
| pubmed:meshHeading |
pubmed-meshheading:15134341-Adenosine Triphosphate,
pubmed-meshheading:15134341-Animals,
pubmed-meshheading:15134341-Animals, Newborn,
pubmed-meshheading:15134341-Cell Hypoxia,
pubmed-meshheading:15134341-Chemoreceptor Cells,
pubmed-meshheading:15134341-Cricetinae,
pubmed-meshheading:15134341-Dose-Response Relationship, Drug,
pubmed-meshheading:15134341-Female,
pubmed-meshheading:15134341-Gene Expression Regulation,
pubmed-meshheading:15134341-Lung,
pubmed-meshheading:15134341-Male,
pubmed-meshheading:15134341-Membrane Potentials,
pubmed-meshheading:15134341-Mesocricetus,
pubmed-meshheading:15134341-Purinergic Agonists,
pubmed-meshheading:15134341-Purinergic Antagonists,
pubmed-meshheading:15134341-Receptors, Purinergic,
pubmed-meshheading:15134341-Respiratory Mucosa,
pubmed-meshheading:15134341-Serotonin
|
| pubmed:articleTitle |
Expression of functional purinergic receptors in pulmonary neuroepithelial bodies and their role in hypoxia chemotransmission.
|
| pubmed:affiliation |
Division of Pathology, Department of Pediatric Laboratory Medicine, The Research Institute, The Hospital for Sick Children and University of Toronto, Toronto, Ontario M5G 1X8, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|