Linked Life Data

15133222

Source:http://linkedlifedata.com/resource/pubmed/id/15133222

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2004-5-10
pubmed:abstractText
An HPLC method using isocratic elution was established for the analysis of fifteen anthocyanins contained in bilberry (Vaccinium myrtillus L.). Separation was attained by using an aqueous solution of 20% methanol containing 0.5% TFA as the mobile phase with a flow rate of 2 ml/min. The detection limit was 0.3 pmol for delphinidin 3-O-beta-D-glucopyranoside, which is a major anthocyanin in bilberry extract. The reproducibility was 0.19-3.85% (S.E.M) for peak area and 0.64-0.77% (S.E.M) for relative mobility normalized by the elution position of the solvent peak. When the relative elution volumes of each anthocyanins were correlated to their corresponding anthocyanin structures, a characteristic pattern was observed. From this pattern, the structures of unknown anthocyanins could be predicted from their elution times. Therefore, the present method is useful for the study of anthocyanins from various biological sources.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0009-2363
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
628-30
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Structural dependence of HPLC separation pattern of anthocyanins from Bilberry (Vaccinium myrtillus L.).
pubmed:affiliation
Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, Japan. kouji@niigata-pharm.ac.jp
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't