| pubmed-article:15133080 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15133080 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:15133080 | lifeskim:mentions | umls-concept:C0005290 | lld:lifeskim |
| pubmed-article:15133080 | lifeskim:mentions | umls-concept:C0085319 | lld:lifeskim |
| pubmed-article:15133080 | lifeskim:mentions | umls-concept:C1533691 | lld:lifeskim |
| pubmed-article:15133080 | lifeskim:mentions | umls-concept:C1519595 | lld:lifeskim |
| pubmed-article:15133080 | pubmed:issue | Pt 5 | lld:pubmed |
| pubmed-article:15133080 | pubmed:dateCreated | 2004-5-10 | lld:pubmed |
| pubmed-article:15133080 | pubmed:abstractText | The genome of Cryptosporidium parvum contains a relatively small number of introns, which includes the beta-tubulin gene with only a single intron. Recently, it was observed that the intron was not removed from some of the beta-tubulin transcripts in the late life cycle stages cultured in vitro. Although normally spliced beta-tubulin mRNA was detected in all parasite intracellular stages by RT-PCR (e.g. HCT-8 or Caco-2 cells infected with C. parvum for 12-72 h), at 48-72 h post-infection unprocessed beta-tubulin transcripts containing intact introns started to appear in parasite mRNA within infected host cells. The intron-containing transcripts could be detected by fluorescence in situ hybridization (FISH) using an intron-specific probe. The intron-containing beta-tubulin transcripts appeared unique to the in vitro-cultured C. parvum, since they were not detected in parasite-infected calves at 72 h. As yet, it is unclear whether the late life cycle stages of C. parvum are partially deficient in intron-splicing or the intron-splicing processes have merely slowed, both of which would allow the detection of intron-containing transcripts. Another possible explanation is that the decay in transcript processing might simply be due to the onset of parasite death. Nonetheless, the appearance of intron-containing transcripts coincides with the arrest of C. parvum development in vitro. This unusual observation prompts speculation that the abnormal intron-splicing of beta-tubulin transcripts may be one of the factors preventing complete development of this parasite in vitro. Furthermore, the presence of both processed and unprocessed introns in beta-tubulin transcripts in vitro may provide a venue for studying overall mechanisms for intron-splicing in this parasite. | lld:pubmed |
| pubmed-article:15133080 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15133080 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15133080 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15133080 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15133080 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15133080 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15133080 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15133080 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15133080 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15133080 | pubmed:month | May | lld:pubmed |
| pubmed-article:15133080 | pubmed:issn | 1350-0872 | lld:pubmed |
| pubmed-article:15133080 | pubmed:author | pubmed-author:HuntN ANA | lld:pubmed |
| pubmed-article:15133080 | pubmed:author | pubmed-author:AbrahamsenMit... | lld:pubmed |
| pubmed-article:15133080 | pubmed:author | pubmed-author:CaiXiaominX | lld:pubmed |
| pubmed-article:15133080 | pubmed:author | pubmed-author:LanctoCheryl... | lld:pubmed |
| pubmed-article:15133080 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15133080 | pubmed:volume | 150 | lld:pubmed |
| pubmed-article:15133080 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15133080 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15133080 | pubmed:pagination | 1191-5 | lld:pubmed |
| pubmed-article:15133080 | pubmed:dateRevised | 2007-11-14 | lld:pubmed |
| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
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| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
| pubmed-article:15133080 | pubmed:meshHeading | pubmed-meshheading:15133080... | lld:pubmed |
| pubmed-article:15133080 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15133080 | pubmed:articleTitle | Intron-containing beta-tubulin transcripts in Cryptosporidium parvum cultured in vitro. | lld:pubmed |
| pubmed-article:15133080 | pubmed:affiliation | Department of Veterinary Pathobiology, College of Veterinary Pathobiology, Texas A&M University, 4467 TAMU, College Station, TX 77843-4467, USA. | lld:pubmed |
| pubmed-article:15133080 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15133080 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15133080 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15133080 | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:15133080 | lld:pubmed |
