Source:http://linkedlifedata.com/resource/pubmed/id/15133080
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
Pt 5
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| pubmed:dateCreated |
2004-5-10
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| pubmed:abstractText |
The genome of Cryptosporidium parvum contains a relatively small number of introns, which includes the beta-tubulin gene with only a single intron. Recently, it was observed that the intron was not removed from some of the beta-tubulin transcripts in the late life cycle stages cultured in vitro. Although normally spliced beta-tubulin mRNA was detected in all parasite intracellular stages by RT-PCR (e.g. HCT-8 or Caco-2 cells infected with C. parvum for 12-72 h), at 48-72 h post-infection unprocessed beta-tubulin transcripts containing intact introns started to appear in parasite mRNA within infected host cells. The intron-containing transcripts could be detected by fluorescence in situ hybridization (FISH) using an intron-specific probe. The intron-containing beta-tubulin transcripts appeared unique to the in vitro-cultured C. parvum, since they were not detected in parasite-infected calves at 72 h. As yet, it is unclear whether the late life cycle stages of C. parvum are partially deficient in intron-splicing or the intron-splicing processes have merely slowed, both of which would allow the detection of intron-containing transcripts. Another possible explanation is that the decay in transcript processing might simply be due to the onset of parasite death. Nonetheless, the appearance of intron-containing transcripts coincides with the arrest of C. parvum development in vitro. This unusual observation prompts speculation that the abnormal intron-splicing of beta-tubulin transcripts may be one of the factors preventing complete development of this parasite in vitro. Furthermore, the presence of both processed and unprocessed introns in beta-tubulin transcripts in vitro may provide a venue for studying overall mechanisms for intron-splicing in this parasite.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
1350-0872
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
150
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1191-5
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| pubmed:dateRevised |
2007-11-14
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| pubmed:meshHeading |
pubmed-meshheading:15133080-Animals,
pubmed-meshheading:15133080-Caco-2 Cells,
pubmed-meshheading:15133080-Cattle,
pubmed-meshheading:15133080-Cattle Diseases,
pubmed-meshheading:15133080-Cell Line,
pubmed-meshheading:15133080-Cryptosporidiosis,
pubmed-meshheading:15133080-Cryptosporidium parvum,
pubmed-meshheading:15133080-Humans,
pubmed-meshheading:15133080-In Situ Hybridization, Fluorescence,
pubmed-meshheading:15133080-Introns,
pubmed-meshheading:15133080-RNA, Messenger,
pubmed-meshheading:15133080-RNA Splicing,
pubmed-meshheading:15133080-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15133080-Transcription, Genetic,
pubmed-meshheading:15133080-Tubulin
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| pubmed:year |
2004
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| pubmed:articleTitle |
Intron-containing beta-tubulin transcripts in Cryptosporidium parvum cultured in vitro.
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| pubmed:affiliation |
Department of Veterinary Pathobiology, College of Veterinary Pathobiology, Texas A&M University, 4467 TAMU, College Station, TX 77843-4467, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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