Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
Pt 1
pubmed:dateCreated
2004-8-6
pubmed:databankReference
pubmed:abstractText
Insects express arthro-series glycosphingolipids, which contain an alpha1,4-linked GalNAc residue. To determine the genetic basis for this linkage, we cloned a cDNA (CG17223) from Drosophila melanogaster encoding a protein with homology to mammalian alpha1,4-glycosyltransferases and expressed it in the yeast Pichia pastoris. Culture supernatants from the transformed yeast were found to display a novel UDP-GalNAc:GalNAcbeta1,4GlcNAcbeta1-R alpha-N-acetylgalactosaminyltransferase activity when using either a glycolipid, p-nitrophenylglycoside or an N-glycan carrying one or two terminal beta-N-acetylgalactosamine residues. NMR and MS in combination with glycosidase digestion and methylation analysis indicate that the cloned cDNA encodes an alpha1,4-N-acetylgalactosaminyltransferase. We hypothesize that this enzyme and its orthologues in other insects are required for the biosynthesis of the N5a and subsequent members of the arthro-series of glycolipids as well as of N-glycan receptors for Bacillus thuringiensis crystal toxin Cry1Ac.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-10430883, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-10583390, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-10747952, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-10748143, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-10816437, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-10848971, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-10854428, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-10993874, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-11443131, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-11530201, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-11714683, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12130631, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12130651, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12167666, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12215432, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12234251, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12244071, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12454022, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12590131, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12655779, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12691742, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12770780, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12888565, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-12944392, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-1483386, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-14871616, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-14976987, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-15006756, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-1628616, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-1889407, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-2351669, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-2384087, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-3988759, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-4042112, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-7540902, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-7686134, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-8139517, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-8206921, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-8557103, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-8631270, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-9012507, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-9334165, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-9417105, http://linkedlifedata.com/resource/pubmed/commentcorrection/15130086-9837949
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
1470-8728
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
382
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
67-74
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
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