Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
2004-5-7
pubmed:abstractText
This work evaluates the influence of different pharmaceutical auxiliaries (Pluronic F68, polyvinylpyrrolidone [PVP] or Tween 20), when mixed with an antigenic extract from Brucella ovis (hot saline; HS), on the characteristics of the resulting poly(epsilon-caprolactone) (PEC) and poly(lactide-co-glycolide) (PLGA) microparticles. In all cases, PEC microparticles were smaller than PLGA ones. Concerning the HS loading, PLGA microparticles were highly dependent on the type of the excipient used, whereas all the PEC formulations displayed similar encapsulation efficiencies. For both types of microparticles, the presence of PVP induced a burst release effect. On the contrary, the use of Tween 20 or Pluronic F68 dramatically modified this profile. For PLGA-Tween 20 and PEC-Pluronic F68 microparticles, the HS was released in a pulsatil way during the first 7 days followed by a continuous release for at least 3 weeks. The antigenicity of the HS components was kept in all cases. Phagocytosis by murine monocytes showed a clear difference based just on the hydrophobicity of the polymer, being PEC microparticles better engulfed. Cell activation quantified by the release of H2O2 did not showed major differences between batches, however, microparticles of PEC and Pluronic F68 induced the highest nitric oxide production. Together, these results confirm the advantageous qualities of the "HS-PEC-Pluronic F68 microparticles" as favorable candidate for vaccine purposes against brucellosis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0378-5173
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-35
pubmed:dateRevised
2008-8-14
pubmed:meshHeading
pubmed-meshheading:15129979-Animals, pubmed-meshheading:15129979-Brucella Vaccine, pubmed-meshheading:15129979-Brucella ovis, pubmed-meshheading:15129979-Brucellosis, pubmed-meshheading:15129979-Electrophoresis, Polyacrylamide Gel, pubmed-meshheading:15129979-Excipients, pubmed-meshheading:15129979-Immunoblotting, pubmed-meshheading:15129979-Lactic Acid, pubmed-meshheading:15129979-Macrophage Activation, pubmed-meshheading:15129979-Macrophages, pubmed-meshheading:15129979-Mice, pubmed-meshheading:15129979-Phagocytosis, pubmed-meshheading:15129979-Poloxamer, pubmed-meshheading:15129979-Polyesters, pubmed-meshheading:15129979-Polyglycolic Acid, pubmed-meshheading:15129979-Polymers, pubmed-meshheading:15129979-Polysorbates, pubmed-meshheading:15129979-Povidone, pubmed-meshheading:15129979-Solubility
pubmed:year
2004
pubmed:articleTitle
Influence of the co-encapsulation of different excipients on the properties of polyester microparticle-based vaccine against brucellosis.
pubmed:affiliation
Department of Pharmaceutical Technology, University of Navarra, 31008 Pamplona, Spain.
pubmed:publicationType
Journal Article, Comparative Study, In Vitro, Research Support, Non-U.S. Gov't