Linked Life Data

15128391

Source:http://linkedlifedata.com/resource/pubmed/id/15128391

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2004-5-6
pubmed:abstractText
Neuronal precursors play an important role in potential regenerative therapeutic strategies in different neurodegenerative diseases, e.g. Parkinson's disease. To understand proliferation and differentiation of these cells in vitro and in vivo, it is important to characterize functional properties of neuronal precursors in detail. The aim of the present study was to analyse the electrophysiological characteristics of ligand-gated channels of neuronal precursors prepared from the rat ventral mesencephalon (VM) of embryonic stage 12.5 during their in vitro differentiation. For the experiments we used the patch-clamp technique in combination with a system for ultrafast solution exchange and immunocytochemistry. It could be shown that functional active AMPA-type glutamate as well as GABA(A) receptor channels are expressed at an early stage of neuronal development. In culture we observed excitatory as well as inhibitory postsynaptic currents (defined by their different kinetics) which correspond to the activation of AMPAergic and GABAergic receptor channels. Two populations of glutamate-activated currents could be differentiated by their different time course of desensitization whereas the time course of resensitization and deactivation was normally distributed in all cells. GABAergic currents could be blocked by bicuculline and their kinetics correspond to that of GABA(A) receptor channel currents. Summarizing the results, in the present study it was shown for the first time that neuronal embryonic precursors of the rat VM express both functional AMPA-type glutamate and functional GABA(A) receptor channels in vitro.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0953-816X
pubmed:author
pubmed:issnType
Print
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2371-6
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:15128391-Animals, pubmed-meshheading:15128391-Cells, Cultured, pubmed-meshheading:15128391-Electric Stimulation, pubmed-meshheading:15128391-Embryo, Mammalian, pubmed-meshheading:15128391-Excitatory Postsynaptic Potentials, pubmed-meshheading:15128391-GABA Antagonists, pubmed-meshheading:15128391-Glutamic Acid, pubmed-meshheading:15128391-Immunohistochemistry, pubmed-meshheading:15128391-Ion Channels, pubmed-meshheading:15128391-Membrane Potentials, pubmed-meshheading:15128391-Mesencephalon, pubmed-meshheading:15128391-Neural Inhibition, pubmed-meshheading:15128391-Neurons, pubmed-meshheading:15128391-Patch-Clamp Techniques, pubmed-meshheading:15128391-Picrotoxin, pubmed-meshheading:15128391-Rats, pubmed-meshheading:15128391-Receptors, AMPA, pubmed-meshheading:15128391-Receptors, GABA-A, pubmed-meshheading:15128391-Receptors, Glutamate, pubmed-meshheading:15128391-Time Factors, pubmed-meshheading:15128391-gamma-Aminobutyric Acid
pubmed:year
2004
pubmed:articleTitle
Ligand-gated channels in early mesencephalic neuronal precursors: immunocytochemical and electrophysiological analysis.
pubmed:affiliation
Department of Neurology, Medizinische Hochschule Hannover, 30623 Hannover, Germany.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't