Linked Life Data

15127288

Source:http://linkedlifedata.com/resource/pubmed/id/15127288

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-6-1
pubmed:abstractText
The intermediate filament protein nestin is predominantly expressed in some stem/progenitor cells and appears to be a useful molecular tool to characterise tumours originating from precursor cells of neuroectodermal and mesenchymal lineages. Leydig cells originate in the adult testis by differentiation from stem cells and express a variety of neural and neuroendocrine markers. The possible expression of the neural stem cell marker nestin in Leydig cells and testicular tumour cells was determined by analysing the patterns of nestin expression in normal and pathological human testes by Western blot and immunohistochemical methods. In normal testis, nestin was found in some vascular endothelial cells, a subset of peritubular spindle-shaped cells and some Leydig cells; spermatogenic and Sertoli cells were unstained. In normal Leydig cells, nestin was distributed in the perinuclear cytoplasm and accumulated in the crystalloids of Reinke with ageing. In non-tumour pathologies (cryptorchidism, impaired spermatogenesis), the seminiferous tubules were immunonegative, whereas hyperplastic Leydig cells showed cytoplasmic immunolabelling. In testicular malignancies, nestin was localised in the Sertoli cells of the seminiferous tubules affected with intratubular germ cell neoplasia, in the hyperplastic Leydig cells associated with these tumours and in some components (mesenchymal and neuroepithelial cells) of teratomas; spermatocytic and non-spermatocytic seminomas were unstained. Some vascular endothelial cells were immunolabelled in all tumour samples. Thus, nestin is expressed in a population of normal and hyperplastic Leydig cells and in Sertoli cells in the presence of intratubular germ-cell neoplasia. Nestin may be a good marker for identifying components of testicular teratomas.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0302-766X
pubmed:author
pubmed:issnType
Print
pubmed:volume
316
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
369-76
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:15127288-Adolescent, pubmed-meshheading:15127288-Adult, pubmed-meshheading:15127288-Aged, pubmed-meshheading:15127288-Antigens, Differentiation, pubmed-meshheading:15127288-Child, pubmed-meshheading:15127288-Ectoderm, pubmed-meshheading:15127288-Endothelial Cells, pubmed-meshheading:15127288-Germ Cells, pubmed-meshheading:15127288-Humans, pubmed-meshheading:15127288-Immunohistochemistry, pubmed-meshheading:15127288-Intermediate Filament Proteins, pubmed-meshheading:15127288-Leydig Cells, pubmed-meshheading:15127288-Male, pubmed-meshheading:15127288-Middle Aged, pubmed-meshheading:15127288-Neoplasms, Germ Cell and Embryonal, pubmed-meshheading:15127288-Nerve Tissue Proteins, pubmed-meshheading:15127288-Seminiferous Tubules, pubmed-meshheading:15127288-Sertoli Cells, pubmed-meshheading:15127288-Stem Cells, pubmed-meshheading:15127288-Teratoma, pubmed-meshheading:15127288-Testicular Neoplasms, pubmed-meshheading:15127288-Tumor Markers, Biological
pubmed:year
2004
pubmed:articleTitle
Nestin, a neuroectodermal stem cell marker molecule, is expressed in Leydig cells of the human testis and in some specific cell types from human testicular tumours.
pubmed:affiliation
Departamento de Biología Celular y Genética, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain. mval.toledo@uah.es
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't