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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
2004-5-5
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pubmed:abstractText |
Recent studies have demonstrated that the malignant Reed-Sternberg cells of Hodgkin's lymphoma (HL) secrete and are responsive to interleukin (IL)-13. We hypothesized that overexpression of a soluble IL-13 decoy receptor (sIL-13Ralpha2) via adenoviral-mediated gene transfer would inhibit IL-13-induced Reed-Sternberg cell proliferation. Western blot and ELISA analysis verified expression of sIL-13Ralpha2 in cell lysates and supernatants of AdsIL-13Ralpha2-transduced COS-7 cells. Treatment of two IL-13-responsive HL-derived cell lines, HDLM-2 and L-1236, with AdsIL-13Ralpha2-conditioned medium, resulted in the inhibition of cell proliferation, and down-regulated the phosphorylation of signal transducer and activator of transcription 6 (STAT6), an important mediator of IL-13 signaling. i.v. delivery of AdsIL-13Ralpha2 in NOD/SCID mice with s.c. implanted HDLM-2 cells delayed tumor onset and growth while enhancing survival compared with control mice. Intratumoral administration of AdsIL-13Ralpha2 led to the regression or stabilization of established tumors and was associated with diminished STAT6 phosphorylation. Our data demonstrate that AdsIL-13Ralpha2 can suppress HL growth in vitro and in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Il13ra1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13 Receptor alpha1...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/STAT6 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Stat6 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0008-5472
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pubmed:author |
pubmed-author:BrayMark RMR,
pubmed-author:ClaudioJaime OJO,
pubmed-author:LiZhihuaZ,
pubmed-author:MakTak WTW,
pubmed-author:Masih-KhanEstherE,
pubmed-author:McCartJ AndreaJA,
pubmed-author:SkinniderBrian FBF,
pubmed-author:StewartA KeithAK,
pubmed-author:TrieuYoungY,
pubmed-author:TrudelSuzanneS,
pubmed-author:WenXiao-YanXY,
pubmed-author:ZhuYuan-XiaoYX
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
64
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3271-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15126369-Adenoviridae,
pubmed-meshheading:15126369-Animals,
pubmed-meshheading:15126369-Apoptosis,
pubmed-meshheading:15126369-COS Cells,
pubmed-meshheading:15126369-Cell Division,
pubmed-meshheading:15126369-Cell Line, Tumor,
pubmed-meshheading:15126369-Cercopithecus aethiops,
pubmed-meshheading:15126369-Female,
pubmed-meshheading:15126369-Gene Therapy,
pubmed-meshheading:15126369-Gene Transfer Techniques,
pubmed-meshheading:15126369-Hodgkin Disease,
pubmed-meshheading:15126369-Interleukin-13 Receptor alpha1 Subunit,
pubmed-meshheading:15126369-Mice,
pubmed-meshheading:15126369-Mice, SCID,
pubmed-meshheading:15126369-Phosphorylation,
pubmed-meshheading:15126369-Receptors, Interleukin,
pubmed-meshheading:15126369-Receptors, Interleukin-13,
pubmed-meshheading:15126369-STAT6 Transcription Factor,
pubmed-meshheading:15126369-Solubility,
pubmed-meshheading:15126369-Trans-Activators
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pubmed:year |
2004
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pubmed:articleTitle |
Soluble interleukin-13Ralpha2 decoy receptor inhibits Hodgkin's lymphoma growth in vitro and in vivo.
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pubmed:affiliation |
Division of Experimental Therapeutics, Toronto General Research Institute, McLaughlin Centre for Molecular Medicine, Toronto, Ontario, Canada.
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pubmed:publicationType |
Journal Article
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