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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-5-4
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pubmed:abstractText |
Hypoxic pulmonary vasoconstriction (HPV) matches lung perfusion with ventilation which tends to optimize pulmonary gas exchange. Investigations using genetically engineered mice represent a promising approach to understand the underlying mechanisms. Our goal was to characterize basic features of HPV in the isolated buffer-perfused and ventilated mouse lung system. HPV was reproducible for several hours when ventilating the lungs with 1% O2 (10 min) alternated with normoxic ventilation periods (21% O2, 15 min). HPV was well elicitable and most constant using Krebs-Henseleit buffer with the addition of hydroxyethylamylopectin as an oncotic agent. Inhibition of both lung NO and prostanoid formation amplified HPV in an over-additive fashion. HPV was higher in BALB/c mive as compared to C57BL/6 mice, and was approximately threefold enhanced under positive pressure ventilation as compared to negative pressure ventilation. A three hour hypoxic ventilation period resulted in a biphasic vasoconstrictor response with loss of posthypoxic vasodilatation. In summary, we have characterised HPV and established an experimental set-up optimized for investigation of the basic mechanisms of HPV in mice.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1569-9048
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
139
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
191-202
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15123002-15-Hydroxy-11 alpha,9...,
pubmed-meshheading:15123002-Amylopectin,
pubmed-meshheading:15123002-Animals,
pubmed-meshheading:15123002-Anoxia,
pubmed-meshheading:15123002-Aspirin,
pubmed-meshheading:15123002-Blood Pressure,
pubmed-meshheading:15123002-Drug Interactions,
pubmed-meshheading:15123002-Enzyme Inhibitors,
pubmed-meshheading:15123002-Mice,
pubmed-meshheading:15123002-Mice, Inbred BALB C,
pubmed-meshheading:15123002-Mice, Inbred C57BL,
pubmed-meshheading:15123002-Oxygen,
pubmed-meshheading:15123002-Pulmonary Artery,
pubmed-meshheading:15123002-Pulmonary Circulation,
pubmed-meshheading:15123002-Pulmonary Gas Exchange,
pubmed-meshheading:15123002-Species Specificity,
pubmed-meshheading:15123002-Tidal Volume,
pubmed-meshheading:15123002-Vasoconstriction,
pubmed-meshheading:15123002-Vasoconstrictor Agents,
pubmed-meshheading:15123002-omega-N-Methylarginine
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pubmed:year |
2004
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pubmed:articleTitle |
Basic features of hypoxic pulmonary vasoconstriction in mice.
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pubmed:affiliation |
Department of Internal Medicine, Justus-Liebig-University Giessen, Klinikstrasse 36, 35392 Giessen, Germany. norbert.weissmann@innere.med.uni-giessen.de
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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