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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-5-26
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pubmed:abstractText |
The requirement of a large amount of high-quality RNA is a major limiting factor for microarray experiments using biopsies. An average microarray experiment requires 10-100 microg of RNA. However, due to their small size, most biopsies do not yield this amount. Several different approaches for RNA amplification in vitro have been described and applied for microarray studies. In most of these, systematic analyses of the potential bias introduced by the enzymatic modifications are lacking.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-10463974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-10748532,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-10918154,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-11680719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-11823860,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12074164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12086591,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12118244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12169536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12172558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12192390,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12445333,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12469123,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12490681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12560512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12610208,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-12670911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-1689846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15119961-3545465
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1471-2164
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
30
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
29
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15119961-Adenocarcinoma,
pubmed-meshheading:15119961-Animals,
pubmed-meshheading:15119961-Breast Neoplasms,
pubmed-meshheading:15119961-Cell Line, Tumor,
pubmed-meshheading:15119961-DNA, Complementary,
pubmed-meshheading:15119961-DNA-Directed RNA Polymerases,
pubmed-meshheading:15119961-Expressed Sequence Tags,
pubmed-meshheading:15119961-Female,
pubmed-meshheading:15119961-Gene Expression Profiling,
pubmed-meshheading:15119961-Humans,
pubmed-meshheading:15119961-Mice,
pubmed-meshheading:15119961-Mice, SCID,
pubmed-meshheading:15119961-Neoplasm Transplantation,
pubmed-meshheading:15119961-Nucleic Acid Amplification Techniques,
pubmed-meshheading:15119961-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:15119961-RNA, Neoplasm,
pubmed-meshheading:15119961-Reproducibility of Results,
pubmed-meshheading:15119961-Viral Proteins
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pubmed:year |
2004
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pubmed:articleTitle |
Systematic analysis of T7 RNA polymerase based in vitro linear RNA amplification for use in microarray experiments.
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pubmed:affiliation |
Department of Molecular Genome Analysis, German Cancer Research Centre, Heidelberg 69120, Germany. joerg.schneider@dkfz.de
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't,
Evaluation Studies
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