Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2004-6-3
pubmed:abstractText
Gallbladder cancer cells are stimulated by hepatocyte growth factor (HGF) in vitro and in vivo. We constructed an adenovirus vector, AdCMV.NK4, carrying the HGF antagonist HGF/NK4 (NK4) and evaluated whether or not this vector can suppress the peritoneal implantation of gallbladder cancer in a novel peritoneal injury mouse model. A human gallbladder cancer cell line (GB-d1) and human peritoneal mesothelial cells infected with the adenovirus vector produced a substantial level of NK4 protein. An invasion of GB-d1 cells was determined by a coculture with AdCMV.NK4-infected human mesothelial cells in vitro. Both the invasion and migration of GB-d1 cells were dramatically inhibited by this vector in a multiplicity of infection (MOI)-dependent manner. GB-d1 cells were intraperitoneally injected into the nude mice with peritoneal injury, followed by either AdCMV.NK4 or a control vector (AdCMV.LacZ). The incidence and the size of the metastatic tumor drastically decreased by AdCMV.NK4 (MOI 100: n=4, P<.0001). Real-time PCR analysis revealed a transient elevation of mouse HGF mRNA expression at the peritoneal injury sites. AdCMV.NK4 has been suggested to induce the inhibition of the implantation and growth of gallbladder cancer cells in vivo through its anti-HGF activity, and the use of NK4 gene transfer could be an effective modality for preventing peritoneal metastasis of gallbladder cancer.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0929-1903
pubmed:author
pubmed:copyrightInfo
Copyright 2004 Nature Publishing Group
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
431-40
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:15118756-Adenoviridae, pubmed-meshheading:15118756-Animals, pubmed-meshheading:15118756-CHO Cells, pubmed-meshheading:15118756-Cell Line, Tumor, pubmed-meshheading:15118756-Coculture Techniques, pubmed-meshheading:15118756-Cricetinae, pubmed-meshheading:15118756-DNA, Complementary, pubmed-meshheading:15118756-Epithelium, pubmed-meshheading:15118756-Gallbladder Neoplasms, pubmed-meshheading:15118756-Gene Therapy, pubmed-meshheading:15118756-Gene Transfer Techniques, pubmed-meshheading:15118756-Genetic Vectors, pubmed-meshheading:15118756-Hepatocyte Growth Factor, pubmed-meshheading:15118756-Humans, pubmed-meshheading:15118756-Mice, pubmed-meshheading:15118756-Mitogens, pubmed-meshheading:15118756-Neoplasm Invasiveness, pubmed-meshheading:15118756-Neoplasm Metastasis, pubmed-meshheading:15118756-Promoter Regions, Genetic, pubmed-meshheading:15118756-RNA, Messenger, pubmed-meshheading:15118756-Recombinant Proteins, pubmed-meshheading:15118756-Reverse Transcriptase Polymerase Chain Reaction, pubmed-meshheading:15118756-Time Factors
pubmed:year
2004
pubmed:articleTitle
Gallbladder cancer treatment using adenovirus expressing the HGF/NK4 gene in a peritoneal implantation model.
pubmed:affiliation
First Department of Surgery, Fukuoka University School of Medicine, Fukuoka 814-0180, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't