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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2004-4-29
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pubmed:abstractText |
We had previously suggested that phosphorylation of proteins by mitochondrial kinases regulate the activity of NADH/CoQ oxidoreductase. Initial data showed that pyruvate dehydrogenase kinase (PDK) and cAMP-dependent protein kinase A (PKA) phosphorylate mitochondrial membrane proteins. Upon phosphorylation with crude PDK, mitochondria appeared to be deficient in NADH/cytochrome c reductase activity associated with increased superoxide production. Conversely, phosphorylation by PKA resulted in increased NADH/cytochrome c reductase activity and decreased superoxide formation. Current data confirms PKA involvement in regulating Complex I activity through phosphorylation of an 18 kDa subunit. Beef heart NADH/ cytochrome c reductase activity increases to 150% of control upon incubation with PKA and ATP-gamma-S. We have cloned the four human isoforms of PDK and purified beef heart Complex I. Incubation of mitochondria with PDK isoforms and ATP did not alter Complex I activity or superoxide production. Radiolabeling of mitochondria and purified Complex I with PDK failed to reveal phosphorylated proteins.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adenosine Triphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP-Dependent Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Electron Transport Complex I,
http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes,
http://linkedlifedata.com/resource/pubmed/chemical/Ketone Oxidoreductases,
http://linkedlifedata.com/resource/pubmed/chemical/NAD,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Superoxides,
http://linkedlifedata.com/resource/pubmed/chemical/pyruvate dehydrogenase (NADP ),
http://linkedlifedata.com/resource/pubmed/chemical/pyruvate dehydrogenase...
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1572-3887
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-32
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:15115179-Adenosine Triphosphate,
pubmed-meshheading:15115179-Amino Acid Sequence,
pubmed-meshheading:15115179-Animals,
pubmed-meshheading:15115179-Cattle,
pubmed-meshheading:15115179-Cyclic AMP-Dependent Protein Kinases,
pubmed-meshheading:15115179-Electron Transport Complex I,
pubmed-meshheading:15115179-Enzyme Activation,
pubmed-meshheading:15115179-Humans,
pubmed-meshheading:15115179-Isoenzymes,
pubmed-meshheading:15115179-Ketone Oxidoreductases,
pubmed-meshheading:15115179-Mitochondria, Heart,
pubmed-meshheading:15115179-Molecular Sequence Data,
pubmed-meshheading:15115179-NAD,
pubmed-meshheading:15115179-Phosphorylation,
pubmed-meshheading:15115179-Protein Kinases,
pubmed-meshheading:15115179-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15115179-Rats,
pubmed-meshheading:15115179-Recombinant Proteins,
pubmed-meshheading:15115179-Sequence Alignment,
pubmed-meshheading:15115179-Superoxides,
pubmed-meshheading:15115179-Swine
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pubmed:year |
2004
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pubmed:articleTitle |
Regulation of NADH/CoQ oxidoreductase: do phosphorylation events affect activity?
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pubmed:affiliation |
Metabolic Research Programme, The Hospital for Sick Children, 555 University Avenue, Toronto M5G 1X8, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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