Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-4-28
pubmed:abstractText
Elevated plasma levels of free fatty acids (FFA) can produce insulin resistance in skeletal muscle tissue and liver and, together with alterations in beta-cell function, this has been referred to as lipotoxicity. This study explores the effects of FFAs on insulin action in rat adipocytes. Cells were incubated 4 or 24 h with or without an unsaturated FFA, oleate or a saturated FFA, palmitate (0.6 and 1.5 mM, respectively). After the culture period, cells were washed and insulin effects on glucose uptake and lipolysis as well as cellular content of insulin signaling proteins (IRS-1, PI3-kinase, PKB and phosphorylated PKB) and the insulin regulated glucose transporter GLUT4 were measured. No significant differences were found in basal or insulin-stimulated glucose uptake in FFA-treated cells compared to control cells, regardless of fatty acid concentration or incubation period. Moreover, there were no significant alterations in the expression of IRS-1, PI3-kinase, PKB and GLUT4 following FFA exposure. Insulin's ability to stimulate PKB phosphorylation was also left intact. Nor did we find any alterations following FFA exposure in basal or cAMP-stimulated lipolysis or in the ability of insulin to inhibit lipolysis. The results indicate that oleate or palmitate does not directly influence insulin action to stimulate glucose uptake and inhibit lipolysis in rat fat cells. Thus, lipotoxicity does not seem to occur in the fat tissue itself.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Glucose, http://linkedlifedata.com/resource/pubmed/chemical/Glucose Transporter Type 4, http://linkedlifedata.com/resource/pubmed/chemical/Insulin, http://linkedlifedata.com/resource/pubmed/chemical/Insulin Receptor Substrate Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Irs1 protein, rat, http://linkedlifedata.com/resource/pubmed/chemical/Monosaccharide Transport Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Oleic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Palmitates, http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt, http://linkedlifedata.com/resource/pubmed/chemical/Slc2a4 protein, rat
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0018-5043
pubmed:author
pubmed:issnType
Print
pubmed:volume
36
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
203-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:15114517-Adipocytes, pubmed-meshheading:15114517-Animals, pubmed-meshheading:15114517-Cells, Cultured, pubmed-meshheading:15114517-Female, pubmed-meshheading:15114517-Glucose, pubmed-meshheading:15114517-Glucose Transporter Type 4, pubmed-meshheading:15114517-Insulin, pubmed-meshheading:15114517-Insulin Receptor Substrate Proteins, pubmed-meshheading:15114517-Lipolysis, pubmed-meshheading:15114517-Male, pubmed-meshheading:15114517-Monosaccharide Transport Proteins, pubmed-meshheading:15114517-Muscle Proteins, pubmed-meshheading:15114517-Oleic Acid, pubmed-meshheading:15114517-Palmitates, pubmed-meshheading:15114517-Phosphatidylinositol 3-Kinases, pubmed-meshheading:15114517-Phosphoproteins, pubmed-meshheading:15114517-Protein-Serine-Threonine Kinases, pubmed-meshheading:15114517-Proto-Oncogene Proteins, pubmed-meshheading:15114517-Proto-Oncogene Proteins c-akt, pubmed-meshheading:15114517-Rats, pubmed-meshheading:15114517-Rats, Sprague-Dawley, pubmed-meshheading:15114517-Signal Transduction
pubmed:year
2004
pubmed:articleTitle
No in vitro effects of fatty acids on glucose uptake, lipolysis or insulin signaling in rat adipocytes.
pubmed:affiliation
Department of Medicine, Umeå University Hospital, Umeå, Sweden.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't