Linked Life Data

1511407

Source:http://linkedlifedata.com/resource/pubmed/id/1511407

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1992-9-29
pubmed:abstractText
The binding of benzo[a]pyrene, 7,12-dimethylbenz[a]anthracene, 3-methylcholanthrene, benz[a]anthracene, dibenz[a,c]anthracene and phenanthrene to calf thymus DNA in vitro in the absence of enzymatic or chemical activation was investigated using the 32P-postlabeling assay. Reactions were performed in the dark or under white light in 1 ml of Tris-HCl buffer (pH 7.5), containing 150 mM KCl, 250 micrograms of DNA and 0.12 nmol-600 nmol of hydrocarbon. Reactions were incubated for 1 h at 37 degrees C and the extent of hydrocarbon:DNA adduct formation was determined. With the exception of phenanthrene, all of the hydrocarbons investigated formed DNA adducts that were easily detected with the 32P-postlabeling assay. The multiplicity and level of hydrocarbon:DNA adducts varied for each hydrocarbon. A dose related increase in adduct formation was observed. Adduct levels ranged from 0.07 to 15.28 adducts per 10(7) nucleotides. Highest adduct levels were detected with 7,12-dimethylbenz[a]anthracene (DMBA) and benzo[a]-pyrene (B[a]P). Hydrocarbon: DNA adduct formation was enhanced when reactions were performed under white light. A comparison of the adduct levels formed from auto-oxidation and enzymatic activation suggests that 0.05 and 0.26% of the adducts detected in the enzymatic activation of B[a]P and DMBA, can be attributed to auto-oxidation, respectively. These data demonstrate that in the absence of enzymatic or chemical activation, polycyclic aromatic hydrocarbons can undergo auto-oxidation in vitro and form hydrocarbon:DNA adducts that are detectable with the 32P-postlabeling assay.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/7,12-dimethylbenz(a)anthracene-DNA..., http://linkedlifedata.com/resource/pubmed/chemical/9,10-Dimethyl-1,2-benzanthracene, http://linkedlifedata.com/resource/pubmed/chemical/Benz(a)Anthracenes, http://linkedlifedata.com/resource/pubmed/chemical/Benzo(a)pyrene, http://linkedlifedata.com/resource/pubmed/chemical/DNA, http://linkedlifedata.com/resource/pubmed/chemical/DNA Adducts, http://linkedlifedata.com/resource/pubmed/chemical/Phosphorus Radioisotopes, http://linkedlifedata.com/resource/pubmed/chemical/Polycyclic Compounds, http://linkedlifedata.com/resource/pubmed/chemical/benz(a)anthracene-DNA adduct, http://linkedlifedata.com/resource/pubmed/chemical/benzo(a)pyrene-DNA adduct, http://linkedlifedata.com/resource/pubmed/chemical/benzotriphenylene
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0304-3835
pubmed:author
pubmed:issnType
Print
pubmed:day
31
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
35-41
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1992
pubmed:articleTitle
Detection of PAH:DNA adducts from auto-oxidation using 32P-postlabeling.
pubmed:affiliation
Rutgers, State University of New Jersey College of Pharmacy, Piscataway 08855-0789.
pubmed:publicationType
Journal Article, In Vitro, Research Support, U.S. Gov't, P.H.S.