Linked Life Data

15114009

Source:http://linkedlifedata.com/resource/pubmed/id/15114009

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2004-4-28
pubmed:abstractText
Modified vaccinia virus Ankara (MVA) is a valuable tool for the expression of recombinant genes used for such purposes as the study of protein functions or characterization of cellular and humoral immune responses. A major advantage of MVA is its clear safety record, and it can be handled under biosafety level 1 conditions. Despite its replication deficiency in human and most mammalian cells, MVA provides high-level gene expression and has proven to be immunogenic when delivering heterologous antigens in animals and humans. This chapter provides state-of-the-art protocols for generation, plaque isolation, molecular characterization, as well as amplification and purification of MVA vector viruses to obtain recombinant viruses for further evaluation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1064-3745
pubmed:author
pubmed:issnType
Print
pubmed:volume
269
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
77-100
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Construction and isolation of recombinant MVA.
pubmed:affiliation
Institute of Molecular Virology, GSF-Research Center and Technical University Munich, Munich, Germany.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't