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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2004-4-27
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pubmed:abstractText |
The electrophysiological properties of cultured tracheal cells (CTCs) were examined in a murine (C57BL/6J), ovalbumin (OVA)-induced model of allergic airway disease (AAD) at early (3-day OVA-aerosol) and peak (10-day OVA-aerosol) periods of inflammation. Transepithelial potential difference, short-circuit current (Isc), and resistance (RT) were lower in CTCs from 10-day OVA-aerosol animals compared to CTCs from naïve mice. In cells cultured for 5 weeks, RT was greater in naive CTCs than in 10-day OVA-aerosol CTCs at all times (P < 0.01). The Isc response to mucosal amiloride (10(-4) mol/L) was increased in CTCs from 10-day OVA-aerosol mice compared to naïve mice (6.0 +/- 0.37 microA/cm2 versus 1.8 +/- 0.56 microA/cm2; P < 0.001) with intermediate values for CTCs from 3-day OVA-aerosol mice. The cAMP-induced increase in Isc was blunted in 10-day OVA-aerosol animals compared to CTCs from naïve mice (9 +/- 12% versus 39 +/- 7%; P < 0.01) with intermediate values for CTCs from 3-day OVA-aerosol mice. There was no difference in mannitol flux in naïve compared to 10-day OVA-aerosol CTCs. Similar results were found using intact tracheas mounted in a perfusion chamber. These data demonstrate changes in airway epithelial cell function in the OVA-induced model of AAD that may contribute to the pathogenesis of airway inflammation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-10362818,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-10409164,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-10669837,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-11509625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-12170028,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-12532084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-1320547,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-1443155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-1534234,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-1616056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-1651662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-1931077,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-2462359,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-2896958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-2934482,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-3317457,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-3372447,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-5049078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-6320697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-6439087,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-7543698,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-7873199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-8292375,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-8631774,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-9311517,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-9423868,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-9739046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-9745362,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-9774978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15111331-9867498
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0002-9440
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
164
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1849-56
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15111331-Animals,
pubmed-meshheading:15111331-Cells, Cultured,
pubmed-meshheading:15111331-Chlorine,
pubmed-meshheading:15111331-Cyclic AMP,
pubmed-meshheading:15111331-Disease Models, Animal,
pubmed-meshheading:15111331-Electrophysiology,
pubmed-meshheading:15111331-Epithelial Cells,
pubmed-meshheading:15111331-Female,
pubmed-meshheading:15111331-Inflammation,
pubmed-meshheading:15111331-Lung Diseases,
pubmed-meshheading:15111331-Mannitol,
pubmed-meshheading:15111331-Membrane Potentials,
pubmed-meshheading:15111331-Mice,
pubmed-meshheading:15111331-Mice, Inbred C57BL,
pubmed-meshheading:15111331-Time Factors,
pubmed-meshheading:15111331-Trachea
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pubmed:year |
2004
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pubmed:articleTitle |
Electrophysiological properties of the airway: epithelium in the murine, ovalbumin model of allergic airway disease.
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pubmed:affiliation |
Department of Pediatrics, University of Connecticut Health Center, Farmington, Connecticut, USA. mclouit@ccmckids.org
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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