Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
2004-7-5
pubmed:abstractText
The use of doubled haploids improves the efficiency of cultivar development in many crops and can be helpful in genetic and molecular studies. The major problem with this approach is the low efficiency of green plant regeneration. We describe here an efficient method for inducing embryos and regenerating green plants directly from isolated microspores of hexaploid triticale (x Triticosecale Wittmack) cv. Bogo. The absence of growth regulators in the induction medium was the most effective condition for the formation of embryo-like structures. The highest induction rates were observed at microspore densities of 1.5x10(5) microspores and 2x10(5) microspores per milliliter. Such cultures produced an average of 54.9 green plants per single donor spike. The frequency of albino plants ranged from 9.3% to 22.9%. Among the green progeny tested, 30.8% were spontaneously doubled haploids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jul
pubmed:issn
0721-7714
pubmed:author
pubmed:issnType
Print
pubmed:volume
22
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
885-93
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Direct embryogenesis and green plant regeneration from isolated microspores of hexaploid triticale (x Triticosecale Wittmack) cv. Bogo.
pubmed:affiliation
Department of Plant Biotechnology and Cytogenetics, Plant Breeding and Acclimatization Institute, 05-870, Radzikow, Blonie, Poland. s.oleszczuk@ihar.edu.pl
pubmed:publicationType
Journal Article