Source:http://linkedlifedata.com/resource/pubmed/id/15107989
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
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pubmed:dateCreated |
2005-4-27
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pubmed:abstractText |
The 2-5A/RNase L pathway belongs to the antiviral system induced by interferon (IFN). RNase L is an inactive endoribonuclease which is activated by 2'-5' oligoadenylate (2-5A) synthesized by 2',5'-oligoadenylate synthetases. Once activated, RNase L cleaves mRNA, inhibiting the protein synthesis, as well as 28S and 18S ribosomal RNA (rRNA), leading to ribosomal inactivation. In this study, we investigate the role of the RNase L pathway as a cell defence mechanism during Varicella-zoster virus (VZV) replication, and the importance of a 68-kDa protein named RNase L inhibitor (RLI), which specifically inhibits RNase L. We demonstrate that the RNase L and RLI transcripts levels remain constant in VZV-infected cells for 24 h and 12 h, respectively, after which they decrease until the end of the viral cycle. VZV does not significantly modulate the protein level of RNase L during the course of infection. Using an rRNA cleavage assay to analyse the RNase L catalytic activity, we demonstrate that VZV replication leads to a minimal cleavage of rRNA. Moreover, the overexpression of RLI in a permissive cell line has no significant effect on the VZV replication. We conclude that RNase L does not constitute a major cell defence mechanism against the VZV infection.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2-5A-dependent ribonuclease,
http://linkedlifedata.com/resource/pubmed/chemical/ABCE1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/ATP-Binding Cassette Transporters,
http://linkedlifedata.com/resource/pubmed/chemical/Chaperonins,
http://linkedlifedata.com/resource/pubmed/chemical/Endoribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Ribosomal
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0300-8584
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
194
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
25-31
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pubmed:dateRevised |
2011-6-17
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pubmed:meshHeading |
pubmed-meshheading:15107989-ATP-Binding Cassette Transporters,
pubmed-meshheading:15107989-Cell Line, Tumor,
pubmed-meshheading:15107989-Chaperonins,
pubmed-meshheading:15107989-Endoribonucleases,
pubmed-meshheading:15107989-Herpesvirus 3, Human,
pubmed-meshheading:15107989-Humans,
pubmed-meshheading:15107989-RNA, Ribosomal,
pubmed-meshheading:15107989-Virus Replication
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pubmed:year |
2005
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pubmed:articleTitle |
Varicella-zoster virus does not significantly induce cell defence mechanism mediated by the 2-5A/RNase L pathway during its replication cycle.
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pubmed:affiliation |
University of Witten/Herdecke, Institute of Microbiology and Virology, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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