|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
1
|
|
pubmed:dateCreated |
2004-4-23
|
|
pubmed:abstractText |
The BRAF gene is mutated in 66% of melanomas and less frequently in various human cancers. More than 80% of these mutations are T to A transversions at nucleotide 1796 (T1796A), leading to a substitution of glutamic acid for valine at amino acid 599 (V599E). We established a new method for rapidly detecting V599E mutations using real-time polymerase chain reaction and melting curve analysis. Furthermore, we examined mutations in gastrointestinal cancer cell lines using this method. We found a mutation in 1 of 12 (8%) colorectal cancer cell lines, but no mutation was detected in 9 gastric cancer cell lines. These results suggest that the BRAF mutation is unlikely to be involved in gastric carcinogenesis.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Feb
|
|
pubmed:issn |
0165-4608
|
|
pubmed:author |
pubmed-author:AragakiJunJ,
pubmed-author:HikibaYohkoY,
pubmed-author:IjichiHideakiH,
pubmed-author:IkenoueTsuneoT,
pubmed-author:KanaiFumihikoF,
pubmed-author:KawabeTakaoT,
pubmed-author:MatsumuraMasayukiM,
pubmed-author:OhtaMikiM,
pubmed-author:OkamotoMakotoM,
pubmed-author:OmataMasaoM,
pubmed-author:TogoGoichiG,
pubmed-author:WatabeHirotsuguH,
pubmed-author:YamajiYutakaY
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
149
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
68-71
|
|
pubmed:dateRevised |
2009-11-19
|
|
pubmed:meshHeading |
pubmed-meshheading:15104286-Amino Acid Substitution,
pubmed-meshheading:15104286-Colorectal Neoplasms,
pubmed-meshheading:15104286-DNA, Neoplasm,
pubmed-meshheading:15104286-DNA Primers,
pubmed-meshheading:15104286-Gastrointestinal Neoplasms,
pubmed-meshheading:15104286-Humans,
pubmed-meshheading:15104286-Mutation,
pubmed-meshheading:15104286-Nucleic Acid Denaturation,
pubmed-meshheading:15104286-Proto-Oncogene Proteins B-raf,
pubmed-meshheading:15104286-Proto-Oncogene Proteins c-raf,
pubmed-meshheading:15104286-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15104286-Tumor Cells, Cultured
|
|
pubmed:year |
2004
|
|
pubmed:articleTitle |
Rapid detection of mutations in the BRAF gene using real-time polymerase chain reaction and melting curve analysis.
|
|
pubmed:affiliation |
Division of Gastroenterology, The Institute for Adult Diseases, Asahi Life Foundation, 1-9-14, Nishi-Shinjuku, Shinjuku-ku, Tokyo 160-0023, Japan. t-ikenoue@asahi-life.or.jp
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
