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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-4-22
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pubmed:abstractText |
Murine T cells do not endogenously upregulate CD80 expression but rather acquire CD80 from antigen presenting cells (APC) during CD28 ligation. Murine CD80+ memory T cells undergo apoptosis in the presence of high levels of antigen while naive CD80+ T cells are capable of acting as APC and T cell:T cell ligation induces anergy and unresponsiveness to antigen rechallenge. Reversing T cell unresponsiveness may be a key factor in the development of immunotherapy strategies for patients with myeloma. We have determined that B7+ T cells (CD80+ or CD86+) are common in patients with myeloma (n = 45), can be either CD4 or CD8, tend to be associated with stable disease and are polyclonal memory T cells (CD45RO). CD80 mRNA expression was present in CD80+ monocytes but not in CD3+ cells with a similar level of CD80 antigen expression. CD80 and CD86 antigen expression was upregulated on B cells but not T cells during incubation with trimeric human CD40 ligand (huCD40LT) + IL-2. Although there was a gradual loss of expression during in vitro culture, CD80+ T cells could be purified for further study. We conclude that B7 expression is common on T cells of patients with myeloma but that this is acquired rather than endogenously produced. B7+ CD45RO+ T cells constitute a population of memory T cells chronically exposed to antigen and warrant further study.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD3,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD45,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/CD86 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA-4 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CTLA4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ctla4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell...
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
1042-8194
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
45
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
363-71
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15101725-Animals,
pubmed-meshheading:15101725-Antigen Presentation,
pubmed-meshheading:15101725-Antigens, CD,
pubmed-meshheading:15101725-Antigens, CD28,
pubmed-meshheading:15101725-Antigens, CD3,
pubmed-meshheading:15101725-Antigens, CD45,
pubmed-meshheading:15101725-Antigens, CD80,
pubmed-meshheading:15101725-Antigens, CD86,
pubmed-meshheading:15101725-Antigens, Differentiation,
pubmed-meshheading:15101725-Apoptosis,
pubmed-meshheading:15101725-CD4-Positive T-Lymphocytes,
pubmed-meshheading:15101725-CD8-Positive T-Lymphocytes,
pubmed-meshheading:15101725-CTLA-4 Antigen,
pubmed-meshheading:15101725-Cell Separation,
pubmed-meshheading:15101725-Cloning, Molecular,
pubmed-meshheading:15101725-DNA, Complementary,
pubmed-meshheading:15101725-Flow Cytometry,
pubmed-meshheading:15101725-Humans,
pubmed-meshheading:15101725-Immunologic Memory,
pubmed-meshheading:15101725-Immunotherapy,
pubmed-meshheading:15101725-Membrane Glycoproteins,
pubmed-meshheading:15101725-Mice,
pubmed-meshheading:15101725-Multiple Myeloma,
pubmed-meshheading:15101725-Phenotype,
pubmed-meshheading:15101725-Polymerase Chain Reaction,
pubmed-meshheading:15101725-RNA, Messenger,
pubmed-meshheading:15101725-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:15101725-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15101725-T-Lymphocytes,
pubmed-meshheading:15101725-Up-Regulation
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pubmed:year |
2004
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pubmed:articleTitle |
B7+ T cells in myeloma: an acquired marker of prior chronic antigen presentation.
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pubmed:affiliation |
Institute of Haematology, Royal Prince Alfred Hospital, Sydney, NSW Australia. rbrown@haem.rpa.cs.nsw.gov.au
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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