Source:http://linkedlifedata.com/resource/pubmed/id/15095273
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|---|---|
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umls-concept:C2349967
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| pubmed:issue |
4
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| pubmed:dateCreated |
2004-4-19
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| pubmed:abstractText |
Head and neck paragangliomas are considered to be biphasic tumours, composed of two distinct cell types: chief cells and sustentacular cells. A substantial number of these tumours show mutations in the SDHD gene located at chromosome 11q23. Although there is general agreement that paragangliomas are a neoplastic proliferation of chief cells, the nature of sustentacular cells is still a matter of debate. To clarify the nature of sustentacular cells further, multiparameter DNA flow cytometry was performed utilizing S-100 labelling as a selective marker of the sustentacular fraction simultaneously with DNA content measurement in six head and neck paragangliomas. S-100-positive fractions and other tumour-cell populations were flow-sorted and restriction digestion analysis of SDHD mutations was performed on each fraction. Flow cytometry demonstrated that the S-100 labelled cells were diploid. Restriction digestion analysis in informative cases revealed retention of the wild-type SDHD allele in S-100-positive fractions and loss of the wild-type allele in S-100-negative fractions. These data strongly suggest that sustentacular cells should be regarded as a non-neoplastic cell population that may be induced as a tumour-specific stromal component by chief cells.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
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| pubmed:issn |
0022-3417
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 2004 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
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| pubmed:issnType |
Print
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| pubmed:volume |
202
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
456-62
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15095273-Allelic Imbalance,
pubmed-meshheading:15095273-DNA, Neoplasm,
pubmed-meshheading:15095273-Diploidy,
pubmed-meshheading:15095273-Flow Cytometry,
pubmed-meshheading:15095273-Head and Neck Neoplasms,
pubmed-meshheading:15095273-Humans,
pubmed-meshheading:15095273-Immunoenzyme Techniques,
pubmed-meshheading:15095273-Loss of Heterozygosity,
pubmed-meshheading:15095273-Neoplasm Proteins,
pubmed-meshheading:15095273-Paraganglioma,
pubmed-meshheading:15095273-S100 Proteins,
pubmed-meshheading:15095273-Succinate Dehydrogenase
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| pubmed:year |
2004
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| pubmed:articleTitle |
Multiparameter DNA flow-sorting demonstrates diploidy and SDHD wild-type gene retention in the sustentacular cell compartment of head and neck paragangliomas: chief cells are the only neoplastic component.
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| pubmed:affiliation |
Department of Pathology, Leiden University Medical Centre, Leiden, The Netherlands.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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