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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-4-19
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pubmed:abstractText |
A recombinant adenoviral tetracycline-regulated system with neuron-specific enolase (NSE) promoter was injected stereotaxically into the striatum of rat brains. The efficiency of in vivo transfection was quantified by counting the number of green fluorescent protein (GFP)-positive cells at 3 days, 1 week, and 4 weeks after injection. NeuN immunohistochemistry demonstrated that expression of gammaPKC-GFP was dominant (20-99%) in neuron and expression of gammaPKC-GFP in neuron was significantly higher in pups than adult rats. These results indicate that tetracycline-inhibitable transcription factor (tTA) can drive tetracycline-responsive promoter (TetOp) under the control of NSE promoter, thereby efficiently and selectively expressing gammaPKC-GFP in neurons in vivo.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
317
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1144-8
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15094388-Adenoviridae,
pubmed-meshheading:15094388-Animals,
pubmed-meshheading:15094388-Animals, Newborn,
pubmed-meshheading:15094388-Brain,
pubmed-meshheading:15094388-Gene Expression,
pubmed-meshheading:15094388-Gene Targeting,
pubmed-meshheading:15094388-Genetic Vectors,
pubmed-meshheading:15094388-Green Fluorescent Proteins,
pubmed-meshheading:15094388-Injections,
pubmed-meshheading:15094388-Luminescent Proteins,
pubmed-meshheading:15094388-Male,
pubmed-meshheading:15094388-Microscopy, Fluorescence,
pubmed-meshheading:15094388-Neurons,
pubmed-meshheading:15094388-Phosphopyruvate Hydratase,
pubmed-meshheading:15094388-Promoter Regions, Genetic,
pubmed-meshheading:15094388-Protein Kinase C,
pubmed-meshheading:15094388-Rats,
pubmed-meshheading:15094388-Rats, Sprague-Dawley,
pubmed-meshheading:15094388-Stereotaxic Techniques,
pubmed-meshheading:15094388-Tetracycline,
pubmed-meshheading:15094388-Transgenes
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pubmed:year |
2004
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pubmed:articleTitle |
In vivo transgene expression using an adenoviral tetracycline-regulated system with neuron-specific enolase promoter.
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pubmed:affiliation |
Department of Neurosurgery, Kobe University Graduate School of Medicine, 7-5-1 Kusunoki Cho, Chuo-Ku, Kobe 650-0017, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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