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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-4-19
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pubmed:abstractText |
Recombination activating gene-1 (RAG-1) and RAG-2 are expressed specifically in lymphocytes undergoing the antigen receptor gene rearrangement during the lymphocyte development. Our previous study showed that the -41 to -17 nucleotides (nt) 5' -upstream region of mouse RAG-2 were pre-requisite for the core promoter activity and that Pax-5/c-Myb/LEF-1 protein-protein complex was responsible for its activity in immature B cells. In this study, we show that the -65/-42 sequence, the non-conserved sequence between human and mouse RAG-2 promoter, is necessary for the full promoter activity for mouse RAG-2. Electrophoresis mobility shift assay revealed that Myc-associated zinc finger protein (MAZ) as well as SP1/3 binds a GA box in this region. Using chromatin immunoprecipitation, we show that MAZ binds the RAG-2 promoter region in pre-B cells. Furthermore, we show that MAZ synergistically activates the murine RAG-2 promoter with Pax-5/c-Myb/LEF-1 complex. These results first demonstrate that MAZ participates in activation of mouse RAG-2 promoter.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myb,
http://linkedlifedata.com/resource/pubmed/chemical/RAG-1 protein,
http://linkedlifedata.com/resource/pubmed/chemical/RAG2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Rag2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/V(D)J recombination activating...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
14
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pubmed:volume |
317
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1096-102
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:15094381-Animals,
pubmed-meshheading:15094381-B-Lymphocyte Subsets,
pubmed-meshheading:15094381-Base Composition,
pubmed-meshheading:15094381-Base Sequence,
pubmed-meshheading:15094381-Cell Line,
pubmed-meshheading:15094381-DNA-Binding Proteins,
pubmed-meshheading:15094381-Gene Expression Regulation,
pubmed-meshheading:15094381-Homeodomain Proteins,
pubmed-meshheading:15094381-Humans,
pubmed-meshheading:15094381-Luciferases,
pubmed-meshheading:15094381-Mice,
pubmed-meshheading:15094381-NIH 3T3 Cells,
pubmed-meshheading:15094381-Nuclear Proteins,
pubmed-meshheading:15094381-Promoter Regions, Genetic,
pubmed-meshheading:15094381-Proto-Oncogene Proteins c-myb,
pubmed-meshheading:15094381-Sequence Alignment,
pubmed-meshheading:15094381-Transcription Factors,
pubmed-meshheading:15094381-Transfection,
pubmed-meshheading:15094381-Zinc Fingers
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pubmed:year |
2004
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pubmed:articleTitle |
Activation of mouse RAG-2 promoter by Myc-associated zinc finger protein.
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pubmed:affiliation |
Department of Immunology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, 2630, Sugitani, Toyama 930-0194, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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