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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
2004-4-19
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pubmed:abstractText |
Here, we developed a binary vector system that introduces a synthetic SUMO-1 conjugation pathway into Escherichia coli and demonstrated that large amounts of sumoylated Ran GTPase activating protein 1 C-terminal region (RanGAP1-C2), Ran binding protein 2 internal repeat domain, p53 and promyelocytic leukemia were efficiently produced. The sumoylated recombinant RanGAP1-C2 appeared to retain the in vivo properties, since it was specifically sumoylated at lysine 517 as expected from in vivo studies. Our findings indicate the establishment of a biosynthetic route for producing large amounts of sumoylated recombinant proteins that will open up new avenues for studying the biochemical and structural aspects of the SUMO-1 modification pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
564
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
85-90
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15094046-Animals,
pubmed-meshheading:15094046-Binding Sites,
pubmed-meshheading:15094046-Cloning, Molecular,
pubmed-meshheading:15094046-Escherichia coli,
pubmed-meshheading:15094046-GTPase-Activating Proteins,
pubmed-meshheading:15094046-Genetic Vectors,
pubmed-meshheading:15094046-Mice,
pubmed-meshheading:15094046-Protein Processing, Post-Translational,
pubmed-meshheading:15094046-Recombinant Proteins,
pubmed-meshheading:15094046-SUMO-1 Protein,
pubmed-meshheading:15094046-Ubiquitin-Activating Enzymes
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pubmed:year |
2004
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pubmed:articleTitle |
Generation of SUMO-1 modified proteins in E. coli: towards understanding the biochemistry/structural biology of the SUMO-1 pathway.
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pubmed:affiliation |
Department of Regeneration Medicine, Institute of Molecular Embryology and Genetics, Kumamoto University, 2-2-1 Honjo, Kumamoto 860-0811, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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