Linked Life Data

15093228

Source:http://linkedlifedata.com/resource/pubmed/id/15093228

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2004-4-19
pubmed:abstractText
Histone H1 conjugated superparamagnetic nanoparticles were assessed for their ability to work as magnetic tracers in conjunction with the relative magnetic permeability metre (MPM-100) for the detection and quantification of DNA (deoxyribonucleic acid). The method employed was based on the electrostatic adsorption of DNA (analyte) to amino group derivatised silica (carrier) and subsequent binding of histone H1 conjugated superparamagnetic nanoparticles (magnetic tracer). The sandwich complexes formed were separated from the medium by sedimentation and the relative magnetic permeability of the sediments were measured with the MPM-100. Investigations were made with both calf thymus DNA and plasmid DNA in aqueous buffered solution as well as in a lysed cell culture with high protein content. For the quantification of calf thymus DNA, a linear relationship between the DNA concentration in the sample and the relative magnetic permeability of the pellet was found for DNA concentrations up to 67 microg/ml in buffered solutions as well as in a lysed cell culture. The limits of detection were determined to 12 and 31 microg/ml, respectively. For the quantification of plasmid DNA in buffered solution a linear range was established for concentrations in up to 150 microg/ml and the limit of detection was determined to 52 microg/ml.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0956-5663
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
19
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1549-57
pubmed:dateRevised
2009-7-14
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
A preliminary study on DNA detection based on relative magnetic permeability measurements and histone H1 conjugated superparamagnetic nanoparticles as magnetic tracers.
pubmed:affiliation
Department of Pure and Applied Biochemistry, Centre for Chemical Engineering, Lund University, P.O. Box 124, SE-221 00 Lund, Sweden.
pubmed:publicationType
Journal Article