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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2004-4-19
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pubmed:abstractText |
We have previously observed the suppression of lung tumor growth in response to overexpression of melanoma differentiation-associated gene-7 (MDA-7)/interleukin-24 (IL-24; approved gene symbol IL24) in vitro and in vivo. MDA-7/IL-24 exerts its tumor-suppressive effects by multiple mechanisms, including the activation of the caspase cascade and the inhibition of angiogenesis. In this study, we used an adenoviral vector (Ad-mda7) to examine the effect of the ectopic production of MDA-7/IL-24 on cell migration and invasion by human non-small-cell lung carcinoma cells. Lung tumor cells (H1299 and A549) treated in vitro with Ad-mda7 migrated and invaded less than cells treated with phosphate-buffered saline (PBS) or Ad-Luc (vector control). MDA-7/IL-24 inhibited migration and invasion by down-regulating the production of phosphatidylinositol 3-kinase/protein kinase B, focal adhesion kinase, and matrix metalloproteinase-2 and -9 relative to PBS and Ad-Luc. Furthermore, tumor cells treated with Ad-mda7 ex vivo or with DOTAP:Chol-mda7 complex in vivo formed significantly fewer tumors in an experimental lung metastasis model. These results show that MDA-7/IL-24 inhibits invasion and migration by lung cancer cells by down-regulating proteins associated with these processes, resulting in reduced metastasis. Thus, Ad-mda7 should be considered a therapeutic agent that can inhibit primary tumor growth and prevent metastasis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caspases,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Kinase 1,
http://linkedlifedata.com/resource/pubmed/chemical/Focal Adhesion Protein-Tyrosine...,
http://linkedlifedata.com/resource/pubmed/chemical/Gelatin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukins,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 2,
http://linkedlifedata.com/resource/pubmed/chemical/Matrix Metalloproteinase 9,
http://linkedlifedata.com/resource/pubmed/chemical/PTK2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositol 3-Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-akt,
http://linkedlifedata.com/resource/pubmed/chemical/interleukin-24
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1525-0016
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
510-8
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:15093181-Adenoviridae,
pubmed-meshheading:15093181-Caspases,
pubmed-meshheading:15093181-Cell Line, Tumor,
pubmed-meshheading:15093181-Cell Movement,
pubmed-meshheading:15093181-Cell Survival,
pubmed-meshheading:15093181-Down-Regulation,
pubmed-meshheading:15093181-Focal Adhesion Kinase 1,
pubmed-meshheading:15093181-Focal Adhesion Protein-Tyrosine Kinases,
pubmed-meshheading:15093181-G0 Phase,
pubmed-meshheading:15093181-Gelatin,
pubmed-meshheading:15093181-Gene Therapy,
pubmed-meshheading:15093181-Gene Transfer Techniques,
pubmed-meshheading:15093181-Genes, Tumor Suppressor,
pubmed-meshheading:15093181-Humans,
pubmed-meshheading:15093181-Immunoblotting,
pubmed-meshheading:15093181-Interleukins,
pubmed-meshheading:15093181-Luciferases,
pubmed-meshheading:15093181-Lung Neoplasms,
pubmed-meshheading:15093181-Matrix Metalloproteinase 2,
pubmed-meshheading:15093181-Matrix Metalloproteinase 9,
pubmed-meshheading:15093181-Neoplasm Invasiveness,
pubmed-meshheading:15093181-Neoplasm Metastasis,
pubmed-meshheading:15093181-Neovascularization, Pathologic,
pubmed-meshheading:15093181-Phosphatidylinositol 3-Kinases,
pubmed-meshheading:15093181-Protein-Serine-Threonine Kinases,
pubmed-meshheading:15093181-Protein-Tyrosine Kinases,
pubmed-meshheading:15093181-Proto-Oncogene Proteins,
pubmed-meshheading:15093181-Proto-Oncogene Proteins c-akt,
pubmed-meshheading:15093181-Time Factors
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pubmed:year |
2004
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pubmed:articleTitle |
Ectopic production of MDA-7/IL-24 inhibits invasion and migration of human lung cancer cells.
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pubmed:affiliation |
Department of Thoracic and Cardiovascular Surgery, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA. rramesh@mdanderson.org
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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