Linked Life Data

15090466

Source:http://linkedlifedata.com/resource/pubmed/id/15090466

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
2004-8-17
pubmed:abstractText
Environmental chemicals are thought to play a role in the etiology of breast cancer, because polycyclic acromatic hydrocarbon (PAH)-DNA adducts are detectable in normal and malignant breast tissues. Peripheral blood lymphocytes (PBLs) from female breast cancer patients were more sensitive to in vitro exposure to benzo[a]pyrene diol epoxide (BPDE) than those from healthy controls. Therefore, we hypothesized that reduced DNA repair is associated with risk of breast cancer in women and the risk may be modulated by polymorphisms of DNA repair genes. In a case-control pilot study, we included 69 previously untreated female breast cancer patients and 79 controls frequency matched to the cases on age and ethnicity. The PBLs were used to measure DNA repair capacity (DRC) by using the host-cell reactivation (HCR) assay with a reporter gene damaged by exposure to 60 micro M BPDE prior to transfection. We also genotyped for two common XPD polymorphisms Lys751Gln and Asp312Asn. We found that the mean DRC level was significantly lower in breast cancer patients (10.1%) than in controls (11.1%) (P = 0.008). Subjects with DRC lower than the median level of controls (11.0%) had >3-fold increased risk (OR = 3.36, 95% CI = 1.15-9.80) for breast cancer than did those with higher DRC after adjustment for age, smoking status and assay-related variables. None of the genotypes was statistically significantly associated with an increased risk of breast cancer, which may be due to the small number of observations in each subgroup. The XPD variant genotypes in general predicted the DRC better in the controls than in the cases, suggesting genetic variants of other DNA repair genes may be involved in these breast cancer patients. These findings suggest that women with reduced DRC may be at an increased risk of developing breast cancer. Large studies are warranted to confirm these preliminary findings.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0143-3334
pubmed:author
pubmed:issnType
Print
pubmed:volume
25
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1695-700
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:15090466-7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide, pubmed-meshheading:15090466-Breast Neoplasms, pubmed-meshheading:15090466-Carcinogens, pubmed-meshheading:15090466-Case-Control Studies, pubmed-meshheading:15090466-DNA Adducts, pubmed-meshheading:15090466-DNA Damage, pubmed-meshheading:15090466-DNA Helicases, pubmed-meshheading:15090466-DNA Repair, pubmed-meshheading:15090466-DNA-Binding Proteins, pubmed-meshheading:15090466-Female, pubmed-meshheading:15090466-Genetic Predisposition to Disease, pubmed-meshheading:15090466-Genotype, pubmed-meshheading:15090466-Humans, pubmed-meshheading:15090466-Middle Aged, pubmed-meshheading:15090466-Polymerase Chain Reaction, pubmed-meshheading:15090466-Polymorphism, Genetic, pubmed-meshheading:15090466-Polymorphism, Restriction Fragment Length, pubmed-meshheading:15090466-Proteins, pubmed-meshheading:15090466-Risk Factors, pubmed-meshheading:15090466-Transcription Factors, pubmed-meshheading:15090466-Xeroderma Pigmentosum Group D Protein
pubmed:year
2004
pubmed:articleTitle
Reduced DNA repair of benzo[a]pyrene diol epoxide-induced adducts and common XPD polymorphisms in breast cancer patients.
pubmed:affiliation
Department of Epidemiology, The University of Texas M. D. Anderson Cancer Center, Houston, TX 77030, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.