Source:http://linkedlifedata.com/resource/pubmed/id/15090248
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2004-4-19
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pubmed:abstractText |
We describe a novel, "mix and read" immunoassay for insulin in biological samples using FMAT. Current commercial assays for insulin require multiple washing steps and can be expensive. The insulin assay described is a simple two-step, time-saving assay and amenable to robotics. The linear response for the fluorometric signal is comparable to that observed using classical ELISA and RIA. A series of mouse plasma samples were tested for insulin levels and yielded results comparable to that measured using a commercial ELISA for insulin.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
1540-658X
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
1
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
521-5
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:15090248-Animals,
pubmed-meshheading:15090248-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:15090248-Fluorometry,
pubmed-meshheading:15090248-Humans,
pubmed-meshheading:15090248-Insulin,
pubmed-meshheading:15090248-Mice,
pubmed-meshheading:15090248-Microchemistry,
pubmed-meshheading:15090248-Rats
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pubmed:year |
2003
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pubmed:articleTitle |
A "mix and read" assay for insulin using fluorometric microvolume assay technology.
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pubmed:affiliation |
Metabolic Disorders-Diabetes, Merck Research Laboratories, Rahway, NJ 07065-0900, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study
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