Source:http://linkedlifedata.com/resource/pubmed/id/15086532
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
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| pubmed:dateCreated |
2004-4-16
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| pubmed:abstractText |
During the past few years several new interacting partners for G protein-coupled receptors (GPCRs) have been discovered, suggesting that the activity of these receptors is more complex than previously anticipated. Recently, candidate G protein-coupled receptor associated sorting protein (GASP-1) has been identified as a novel interacting partner for the delta opioid receptor and has been proposed to determine the degradative fate of this receptor. We show here that GASP-1 associates in vitro with other opioid receptors and that the interaction domain in these receptors is restricted to a small portion of the carboxyl-terminal tail, corresponding to helix 8 in the three-dimensional structure of rhodopsin. In addition, we show that GASP-1 interacts with COOH-terminus of several other GPCRs from subfamilies A and B and that two conserved residues within the putative helix 8 of these receptors are critical for the interaction with GASP-1. In situ hybridization and northern blot analysis indicate that GASP-1 mRNA is mainly distributed throughout the central nervous system, consistent with a potential interaction with numerous GPCRs in vivo. Finally, we show that GASP-1 is a member of a novel family comprising at least 10 members, whose genes are clustered on chromosome X. Another member of the family, GASP-2, also interacts with the carboxyl-terminal tail of several GPCRs. Therefore, GASP proteins may represent an important protein family regulating GPCR physiology.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/GPRASP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, delta,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Opioid, mu,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Vesicular Transport Proteins
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| pubmed:status |
MEDLINE
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| pubmed:month |
May
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| pubmed:issn |
0022-3042
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:volume |
89
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
766-75
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:15086532-Amino Acid Sequence,
pubmed-meshheading:15086532-Animals,
pubmed-meshheading:15086532-Central Nervous System,
pubmed-meshheading:15086532-Chromosomes, Human, Pair 10,
pubmed-meshheading:15086532-Cloning, Molecular,
pubmed-meshheading:15086532-Conserved Sequence,
pubmed-meshheading:15086532-Humans,
pubmed-meshheading:15086532-Mice,
pubmed-meshheading:15086532-Molecular Sequence Data,
pubmed-meshheading:15086532-Multigene Family,
pubmed-meshheading:15086532-Organ Specificity,
pubmed-meshheading:15086532-Protein Binding,
pubmed-meshheading:15086532-Protein Transport,
pubmed-meshheading:15086532-RNA, Messenger,
pubmed-meshheading:15086532-Receptors, G-Protein-Coupled,
pubmed-meshheading:15086532-Receptors, Opioid, delta,
pubmed-meshheading:15086532-Receptors, Opioid, mu,
pubmed-meshheading:15086532-Recombinant Fusion Proteins,
pubmed-meshheading:15086532-Sequence Homology, Amino Acid,
pubmed-meshheading:15086532-Two-Hybrid System Techniques,
pubmed-meshheading:15086532-Vesicular Transport Proteins
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| pubmed:year |
2004
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| pubmed:articleTitle |
Identification of a novel family of G protein-coupled receptor associated sorting proteins.
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| pubmed:affiliation |
Institut de Génétique et de Biologie Moléculaire et Cellulaire, CNRS/INSERM/ULP, Illkirch, C. U. de Strasbourg, France. simonin@igbmc.u-strasbg.fr
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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