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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2004-4-16
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pubmed:abstractText |
Toll-like receptors (TLRs) are a recently described family of immune receptors involved in the recognition of pathogen-associated molecular patterns (PAMPs). The central role of TLR-2 and TLR-4 in microbial responses suggests they may be implicated in the pathogenesis of human sepsis. We hypothesized that the incidence and outcome of sepsis would be influenced by the expression of TLR-2 and TLR-4 on monocytes. We have examined the expression of TLR-2 and TLR-4 mRNA and protein and their response to pro- and anti-inflammatory agents on monocytes from subjects in the intensive therapy unit (ITU) with and without Gram-negative, Gram-positive or polymicrobial sepsis. We compared these data to ITU and healthy control subjects. TLR-2 mRNA was significantly up-regulated on monocytes from subjects with both Gram-positive and Gram-negative sepsis. Similarly, we detected increased levels of TLR-2 protein on the surface of monocytes from sepsis subjects relative to ITU controls. TLR-4 mRNA was increased in Gram-positive subjects; however, there was no corresponding increase in TLR-4 protein. Although TLR-4 mRNA expression in healthy control monocytes could be modulated in vitro by culture with lipopolysaccharide or interleukin-10, this was not observed in monocytes obtained from sepsis and ITU control subjects, suggesting that septic and ITU control milieus may alter the immunoregulation of TLR-4 mRNA expression on monocytes. TLR-2 mRNA was not modulated in culture by any stimulus in any group. We suggest that expression and regulatory response of monocyte TLR-2, and to a lesser extent TLR-4 may be abnormal in human sepsis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-10196138,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-10225915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-10548109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-10559223,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-10725699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-10786961,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-10820283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11044374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11123299,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11160242,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11191641,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11261796,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11404392,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11672593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11964313,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-11971020,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-12023360,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-12133792,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-12700374,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-12752109,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-12794424,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-14568981,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-7768603,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-7907683,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-8674326,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-9176536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/15086396-9734942
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/TLR2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/TLR4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 2,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptors
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
136
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
312-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:15086396-Adult,
pubmed-meshheading:15086396-Aged,
pubmed-meshheading:15086396-Aged, 80 and over,
pubmed-meshheading:15086396-Case-Control Studies,
pubmed-meshheading:15086396-Female,
pubmed-meshheading:15086396-Flow Cytometry,
pubmed-meshheading:15086396-Humans,
pubmed-meshheading:15086396-Male,
pubmed-meshheading:15086396-Membrane Glycoproteins,
pubmed-meshheading:15086396-Middle Aged,
pubmed-meshheading:15086396-Monocytes,
pubmed-meshheading:15086396-RNA, Messenger,
pubmed-meshheading:15086396-Receptors, Cell Surface,
pubmed-meshheading:15086396-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:15086396-Sepsis,
pubmed-meshheading:15086396-Statistics, Nonparametric,
pubmed-meshheading:15086396-Toll-Like Receptor 2,
pubmed-meshheading:15086396-Toll-Like Receptor 4,
pubmed-meshheading:15086396-Toll-Like Receptors
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pubmed:year |
2004
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pubmed:articleTitle |
Differential expression of Toll-like receptor (TLR)-2 and TLR-4 on monocytes in human sepsis.
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pubmed:affiliation |
Lung Research Group, University of Bristol Division of Medicine, Southmead Hospital, Bristol, UK. lynne.armstrong@bris.ac.uk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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