Linked Life Data

15082342

Source:http://linkedlifedata.com/resource/pubmed/id/15082342

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2004-4-14
pubmed:abstractText
Apoptotic cell death, phagocytic uptake, and their interplay may induce/trigger autoantibody production and autoimmunity. Herein the role of immunoglobulin G (IgG) anti-dsDNA autoantibodies in nucleo-phagocytosis by polymorpho-nuclear cells (PMN) has been analyzed. Necrotaxis, phagocytosis and PMN with engulfed nuclei can be imaged by vital microscopy. Vital microscopy revealed dead neutrophils with persistence of nuclei or ingested nuclei in 8/10 patients with lupus erythematosus (LE) and in 4/20 healthy controls (P < 0.0006). The phagocytic clearance of dead cells/nuclei did not correspond to any of the analyzed apoptosis markers (annexin V, CD95, and active caspase 3). IgG staining of isolated PBMC nuclei as detected by flow cytometry was significantly higher in parallel to NUC+ (P < 0.02). In vitro phagocytosis of substrates pre-treated with anti-dsDNA containing serum induced an increased phagocytic capacity as compared to normal serum. These data suggest that anti-dsDNA antibodies seem to inhibit enzymatic cleavage of nuclei from damaged cells, and improve their phagocytic uptake by PMN, which finally results in LE cell formation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0753-3322
pubmed:author
pubmed:issnType
Print
pubmed:volume
58
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
196-201
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
LE cell phenomenon: nuclear IgG deposits inhibit enzymatic cleavage of the nucleus of damaged cells and support its phagocytic clearance by PMN.
pubmed:affiliation
Department of Radiology, University of Bonn, Sigmund-Freud Strasse 25, 53105 Bonn, Germany. i.boehm@uni-bonn.de
pubmed:publicationType
Journal Article