Linked Life Data

15081997

Source:http://linkedlifedata.com/resource/pubmed/id/15081997

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2004-4-14
pubmed:abstractText
The biochemistry of piezophilic bacteria is unique in that piezophiles produce polyunsaturated fatty acids (PUFAs). A pertinent question is if piezophilic bacteria synthesize PUFA de novo, through dietary uptake, or both. This study was undertaken to examine the biosynthesis and cellular uptake of PUFAs by piezophilic bacteria. A moderately piezophilic (Shewanella violacea DSS12) and two hyperpiezophilic bacteria (S. benthica DB21MT-2 and Moritella yayanosii DB21MT-5) were grown under 50 MPa (megapascal) and 100 MPa, respectively, in media containing marine broth 2216 supplemented with arachidonic acid (AA, sodium salt) and/or antibiotic cerulenin. There was active uptake and cellular incorporation of AA in the hyperpiezophilic bacteria DB21MT-2 (14.7% of total fatty acids) and DB21MT-5 (1.4%), but no uptake was observed in DSS12. When cells were treated with cerulenin, all three strains incorporated AA into cell membranes (13-19%). The biosynthesis of monounsaturated fatty acids was significantly inhibited (10-37%) by the addition of cerulenin, whereas the concentrations of PUFAs increased by 2-4 times. These results suggest that piezophilic bacteria biosynthesize and/or incorporate dietary polyunsaturated fatty acids that are important for their growth and piezoadaptation. The significance of these findings is also discussed in the context of phenotypic classification of piezophiles.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1096-4959
pubmed:author
pubmed:issnType
Print
pubmed:volume
137
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
455-61
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
Biosynthesis and dietary uptake of polyunsaturated fatty acids by piezophilic bacteria.
pubmed:affiliation
Department of Geological and Atmospheric Sciences, Iowa State University, 360 Science I, Ames, IA 50011, USA. jsfang@iastate.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, Non-P.H.S.