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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017638,
umls-concept:C0079419,
umls-concept:C0162638,
umls-concept:C0205263,
umls-concept:C0249197,
umls-concept:C0288472,
umls-concept:C0683461,
umls-concept:C1420626,
umls-concept:C1514559,
umls-concept:C1517339,
umls-concept:C1704259,
umls-concept:C1705987
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pubmed:issue |
3
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pubmed:dateCreated |
2004-4-14
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pubmed:abstractText |
The regulator of fibroblast growth factor 2 (FGF-2) transcription (RFT) has been reported to be a transcriptional repressor of FGF-2 and induce glioma cell death by its overexpression. Here we report that RFT regulates cell cycle as well as apoptosis by a novel mechanism. RFT expressed in some glioma cell lines, U138MG and T98G, but neither in U87MG nor U251MG. Overexpressed RFT-induced apoptosis in U87MG and U138MG with functioning-type p53 but neither in U251MG nor T98G with non-functioning-type p53. Administration of FGF-2 failed to prevent RFT-induced apoptosis. Overexpression of RFT caused G1-S arrest and upregulated both the phosphorylation of p53 at Ser-15 and the expression level of p21(Waf1). Furthermore, RNAi knockdown of p53 abolished RFT-induced apoptosis in U87MG. Taken together, our results support that RFT regulates G1-S transition and apoptosis via p53/p21(Waf1) pathway.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CDKN1A protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase Inhibitor...,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/MBD1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
7
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pubmed:volume |
317
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
902-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:15081425-Apoptosis,
pubmed-meshheading:15081425-Base Sequence,
pubmed-meshheading:15081425-Brain Neoplasms,
pubmed-meshheading:15081425-Cell Line, Tumor,
pubmed-meshheading:15081425-Cyclin-Dependent Kinase Inhibitor p21,
pubmed-meshheading:15081425-Cyclins,
pubmed-meshheading:15081425-DNA Primers,
pubmed-meshheading:15081425-DNA-Binding Proteins,
pubmed-meshheading:15081425-Fluorescent Antibody Technique,
pubmed-meshheading:15081425-G1 Phase,
pubmed-meshheading:15081425-Glioma,
pubmed-meshheading:15081425-Humans,
pubmed-meshheading:15081425-Molecular Sequence Data,
pubmed-meshheading:15081425-Phosphorylation,
pubmed-meshheading:15081425-Repressor Proteins,
pubmed-meshheading:15081425-S Phase,
pubmed-meshheading:15081425-Transcription Factors,
pubmed-meshheading:15081425-Tumor Suppressor Protein p53
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pubmed:year |
2004
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pubmed:articleTitle |
Overexpression of RFT induces G1-S arrest and apoptosis via p53/p21(Waf1) pathway in glioma cell.
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pubmed:affiliation |
Department of Neurosurgery, Kyoto University Graduate School of Medicine, Sakyo-ku, Kyoto 606-8315, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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