Source:http://linkedlifedata.com/resource/pubmed/id/15081219
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
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| pubmed:dateCreated |
2004-4-14
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| pubmed:abstractText |
A novel pH-sensitive hydrogel system composed of a water-soluble chitosan derivative (N,O-carboxymethyl chitosan, NOCC) and alginate blended with genipin was developed for controlling protein drug delivery. Genipin, a naturally occurring cross-linking agent, is significantly less cytotoxic than glutaraldehyde and may provide a less extent of cross-linking to form a semiinterpenetrating polymeric network (semi-IPN) within the developed hydrogel system. The drug-loading process used in the study was simple and mild. All procedures used were performed in aqueous medium at neutral environment. In the study, preparation of the NOCC/alginate-based hydrogels was reported. Swelling characteristics of these hydrogels as a function of pH values were investigated. Additionally, release profiles of a model protein drug (bovine serum albumin, BSA) from test hydrogels were studied in simulated gastric and intestinal media. The semi-IPN formation of the genipin-cross-linked NOCC/alginate hydrogel was confirmed by means of the scanning electron microscopy-energy dispersive X-ray spectrometer (SEM-EDS) and the ninhydrin assays. The percentage of decrease of free amino groups and cross-linking density for the NOCC/alginate hydrogel cross-linked with 0.75 mM genipin were 18% and 26 mol/m(3), respectively. At pH 1.2, the swelling ratio of the genipin-cross-linked NOCC/alginate hydrogel was limited (2.5) due to formation of hydrogen bonds between NOCC and alginate. At pH 7.4, the carboxylic acid groups on the genipin-cross-linked NOCC/alginate hydrogel became progressively ionized. In this case, the hydrogel swelled more significantly (6.5) due to a large swelling force created by the electrostatic repulsion between the ionized acid groups. The amount of BSA released at pH 1.2 was relatively low (20%), while that released at pH 7.4 increased significantly (80%). The results clearly suggested that the genipin-cross-linked NOCC/alginate hydrogel could be a suitable polymeric carrier for site-specific protein drug delivery in the intestine.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alginates,
http://linkedlifedata.com/resource/pubmed/chemical/Chitosan,
http://linkedlifedata.com/resource/pubmed/chemical/Cross-Linking Reagents,
http://linkedlifedata.com/resource/pubmed/chemical/Delayed-Action Preparations,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Glucuronic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Hexuronic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Hydrogels,
http://linkedlifedata.com/resource/pubmed/chemical/Iridoid Glycosides,
http://linkedlifedata.com/resource/pubmed/chemical/Iridoids,
http://linkedlifedata.com/resource/pubmed/chemical/O,N-carboxymethylchitosan,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pyrans,
http://linkedlifedata.com/resource/pubmed/chemical/Serum Albumin, Bovine,
http://linkedlifedata.com/resource/pubmed/chemical/alginic acid,
http://linkedlifedata.com/resource/pubmed/chemical/genipin
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| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
0168-3659
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
28
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| pubmed:volume |
96
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
285-300
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| pubmed:dateRevised |
2010-11-18
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| pubmed:meshHeading |
pubmed-meshheading:15081219-Alginates,
pubmed-meshheading:15081219-Chitosan,
pubmed-meshheading:15081219-Cross-Linking Reagents,
pubmed-meshheading:15081219-Delayed-Action Preparations,
pubmed-meshheading:15081219-Drug Carriers,
pubmed-meshheading:15081219-Glucuronic Acid,
pubmed-meshheading:15081219-Hexuronic Acids,
pubmed-meshheading:15081219-Hydrogels,
pubmed-meshheading:15081219-Hydrogen Bonding,
pubmed-meshheading:15081219-Hydrogen-Ion Concentration,
pubmed-meshheading:15081219-Iridoid Glycosides,
pubmed-meshheading:15081219-Iridoids,
pubmed-meshheading:15081219-Magnetic Resonance Spectroscopy,
pubmed-meshheading:15081219-Microscopy, Electron, Scanning,
pubmed-meshheading:15081219-Proteins,
pubmed-meshheading:15081219-Pyrans,
pubmed-meshheading:15081219-Serum Albumin, Bovine,
pubmed-meshheading:15081219-Spectroscopy, Fourier Transform Infrared,
pubmed-meshheading:15081219-Time Factors
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| pubmed:year |
2004
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| pubmed:articleTitle |
A novel pH-sensitive hydrogel composed of N,O-carboxymethyl chitosan and alginate cross-linked by genipin for protein drug delivery.
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| pubmed:affiliation |
Department of Chemical Engineering, National Tsing Hua University, 101 Section 2, Kung-Fu Road, Hsinchu 30013, Taiwan, ROC.
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| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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