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rdf:type |
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|
lifeskim:mentions |
|
|
pubmed:issue |
15
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pubmed:dateCreated |
2004-4-14
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|
pubmed:abstractText |
An enhanced sensitivity zero-quantum correlation experiment is proposed for recording (1)H-(13)C correlations of methyl groups in highly deuterated, methyl protonated large proteins. The zero-quantum spectra benefit from TROSY-effects in which both intra- and inter-methyl dipolar relaxation interactions are minimized. Applications to malate synthase G at 5 degrees C (81 kDa single polypeptide chain enzyme, correlation time of 118 ns) and lysine decarboxylase at 45 degrees C (810 kDa decameric enzyme) are presented showing significant improvements in resolution relative to corresponding HMQC data sets, with only slight decreases (approximately 10%) in sensitivity.
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pubmed:language |
eng
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pubmed:journal |
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|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:month |
Apr
|
|
pubmed:issn |
0002-7863
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pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:day |
21
|
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pubmed:volume |
126
|
|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
4921-5
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
|
|
pubmed:year |
2004
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|
pubmed:articleTitle |
Line narrowing in methyl-TROSY using zero-quantum 1H-13C NMR spectroscopy.
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|
pubmed:affiliation |
Protein Engineering Network Centers of Excellence and Department of Medical Genetics, The University of Toronto, Toronto, Ontario, Canada, M5S 1A8.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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