Linked Life Data

15078330

Source:http://linkedlifedata.com/resource/pubmed/id/15078330

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2004-4-13
pubmed:abstractText
Summary Two interacting three amino acid loop extension (TALE) proteins of potato, StBEL5 (Solanum tuberosom BEL 5) and POTH1 (potato homeobox 1), mediate developmental processes by regulating phytohormone levels. Overexpression of either partner alone increased tuber yields by lowering gibberellin (GA) levels and increasing cytokinins. Gel shift assays demonstrated that StBEL5 and POTH1 bind to the regulatory region of ga20 oxidase1 (ga20ox1) from potato, a gene encoding a key enzyme in the GA biosynthetic pathway. In tandem, StBEL5 and POTH1 had a greater binding affinity for the ga20ox1 promoter than either protein alone. The StBEL5-POTH1 heterodimer bound specifically to a composite 10-bp sequence, containing two TGAC cores. Using a transcription assay, StBEL5 and POTH1 suppressed the activity of the ga20ox1 promoter by more than 50%. Dominant negative constructs containing the protein-binding domains of StBEL5 or POTH1 blocked the repression activity of StBEL5 or POTH1, respectively. The mutated ga20ox1 promoter that could be bound by the StBEL5 or POTH1 proteins individually but not by the StBEL5-POTH1 heterodimer also abolished the repression activity of StBEL5, POTH1, and the StBEL5-POTH1 heterodimer. These results indicate that the tandem interaction of StBEL5 and POTH1 is essential for regulation of the expression of their target gene.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0960-7412
pubmed:author
pubmed:issnType
Print
pubmed:volume
38
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
276-84
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
2004
pubmed:articleTitle
The tandem complex of BEL and KNOX partners is required for transcriptional repression of ga20ox1.
pubmed:affiliation
Interdepartmental Plant Physiology Major, Iowa State University, Ames, IA 50011-1100, USA.
pubmed:publicationType
Journal Article