| pubmed-article:15077123 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:15077123 | lifeskim:mentions | umls-concept:C0332307 | lld:lifeskim |
| pubmed-article:15077123 | lifeskim:mentions | umls-concept:C0221908 | lld:lifeskim |
| pubmed-article:15077123 | lifeskim:mentions | umls-concept:C1519715 | lld:lifeskim |
| pubmed-article:15077123 | lifeskim:mentions | umls-concept:C1882727 | lld:lifeskim |
| pubmed-article:15077123 | lifeskim:mentions | umls-concept:C0204727 | lld:lifeskim |
| pubmed-article:15077123 | lifeskim:mentions | umls-concept:C0205409 | lld:lifeskim |
| pubmed-article:15077123 | lifeskim:mentions | umls-concept:C1440080 | lld:lifeskim |
| pubmed-article:15077123 | lifeskim:mentions | umls-concept:C0205250 | lld:lifeskim |
| pubmed-article:15077123 | pubmed:issue | 6 | lld:pubmed |
| pubmed-article:15077123 | pubmed:dateCreated | 2004-5-21 | lld:pubmed |
| pubmed-article:15077123 | pubmed:abstractText | There are no ideal cell lines available for alveolar epithelial type I and II cells (AEC I and II) at the present time. The current methods for isolating AEC I and II give limited purities. Here, we reported improved and reproducible methods for the isolation of highly pure AEC I and II from rat lungs. AEC I and II were released from lung tissues using different concentrations of elastase digestion. Macrophages and leukocytes were removed by rat IgG 'panning' and anti-rat leukocyte common antigen antibodies. For AEC II isolation, polyclonal rabbit anti-T1alpha (an AEC I apical membrane protein) antibodies were used to remove AEC I contamination. For AEC I isolation, positive immunomagnetic selection by polyclonal anti-T1alpha antibodies was used. The purities of AEC I and II were 91 +/- 4 and 97 +/- 1%, respectively. The yield per rat was approximately 2 x 10(6) for AEC I and approximately 33 x 10(6) for AEC II. The viabilities of these cell preparations were more than 96%. The protocol for AEC II isolation is also suitable to obtain pure AEC II (93-95%) from hyperoxia-injured and recovering lungs. The purified AEC I and II can be used for gene expression profiling and functional studies. It also offers an important tool to the field of lung biology. | lld:pubmed |
| pubmed-article:15077123 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:grant | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:language | eng | lld:pubmed |
| pubmed-article:15077123 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:15077123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:15077123 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:15077123 | pubmed:month | Jun | lld:pubmed |
| pubmed-article:15077123 | pubmed:issn | 0023-6837 | lld:pubmed |
| pubmed-article:15077123 | pubmed:author | pubmed-author:AHH | lld:pubmed |
| pubmed-article:15077123 | pubmed:author | pubmed-author:ChenJiwangJ | lld:pubmed |
| pubmed-article:15077123 | pubmed:author | pubmed-author:JinNiliN | lld:pubmed |
| pubmed-article:15077123 | pubmed:author | pubmed-author:ChenZhongming... | lld:pubmed |
| pubmed-article:15077123 | pubmed:author | pubmed-author:NarasarajuTel... | lld:pubmed |
| pubmed-article:15077123 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:15077123 | pubmed:volume | 84 | lld:pubmed |
| pubmed-article:15077123 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:15077123 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:15077123 | pubmed:pagination | 727-35 | lld:pubmed |
| pubmed-article:15077123 | pubmed:dateRevised | 2008-11-21 | lld:pubmed |
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| pubmed-article:15077123 | pubmed:meshHeading | pubmed-meshheading:15077123... | lld:pubmed |
| pubmed-article:15077123 | pubmed:meshHeading | pubmed-meshheading:15077123... | lld:pubmed |
| pubmed-article:15077123 | pubmed:meshHeading | pubmed-meshheading:15077123... | lld:pubmed |
| pubmed-article:15077123 | pubmed:meshHeading | pubmed-meshheading:15077123... | lld:pubmed |
| pubmed-article:15077123 | pubmed:year | 2004 | lld:pubmed |
| pubmed-article:15077123 | pubmed:articleTitle | Isolation of highly pure alveolar epithelial type I and type II cells from rat lungs. | lld:pubmed |
| pubmed-article:15077123 | pubmed:affiliation | Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA. | lld:pubmed |
| pubmed-article:15077123 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:15077123 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:15077123 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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