Source:http://linkedlifedata.com/resource/pubmed/id/15077123
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
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| pubmed:dateCreated |
2004-5-21
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| pubmed:abstractText |
There are no ideal cell lines available for alveolar epithelial type I and II cells (AEC I and II) at the present time. The current methods for isolating AEC I and II give limited purities. Here, we reported improved and reproducible methods for the isolation of highly pure AEC I and II from rat lungs. AEC I and II were released from lung tissues using different concentrations of elastase digestion. Macrophages and leukocytes were removed by rat IgG 'panning' and anti-rat leukocyte common antigen antibodies. For AEC II isolation, polyclonal rabbit anti-T1alpha (an AEC I apical membrane protein) antibodies were used to remove AEC I contamination. For AEC I isolation, positive immunomagnetic selection by polyclonal anti-T1alpha antibodies was used. The purities of AEC I and II were 91 +/- 4 and 97 +/- 1%, respectively. The yield per rat was approximately 2 x 10(6) for AEC I and approximately 33 x 10(6) for AEC II. The viabilities of these cell preparations were more than 96%. The protocol for AEC II isolation is also suitable to obtain pure AEC II (93-95%) from hyperoxia-injured and recovering lungs. The purified AEC I and II can be used for gene expression profiling and functional studies. It also offers an important tool to the field of lung biology.
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| pubmed:grant | |
| pubmed:commentsCorrections | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Gp38 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Pancreatic Elastase,
http://linkedlifedata.com/resource/pubmed/chemical/Pulmonary Surfactant-Associated...
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jun
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| pubmed:issn |
0023-6837
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
84
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
727-35
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:15077123-Animals,
pubmed-meshheading:15077123-Antibodies,
pubmed-meshheading:15077123-Cell Separation,
pubmed-meshheading:15077123-Cell Survival,
pubmed-meshheading:15077123-Epithelial Cells,
pubmed-meshheading:15077123-Hyperoxia,
pubmed-meshheading:15077123-Immunomagnetic Separation,
pubmed-meshheading:15077123-Lung Injury,
pubmed-meshheading:15077123-Male,
pubmed-meshheading:15077123-Membrane Glycoproteins,
pubmed-meshheading:15077123-Membrane Proteins,
pubmed-meshheading:15077123-Microscopy, Electron,
pubmed-meshheading:15077123-Pancreatic Elastase,
pubmed-meshheading:15077123-Pulmonary Alveoli,
pubmed-meshheading:15077123-Pulmonary Surfactant-Associated Proteins,
pubmed-meshheading:15077123-Rats,
pubmed-meshheading:15077123-Rats, Sprague-Dawley,
pubmed-meshheading:15077123-Reproducibility of Results
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| pubmed:year |
2004
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| pubmed:articleTitle |
Isolation of highly pure alveolar epithelial type I and type II cells from rat lungs.
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| pubmed:affiliation |
Department of Physiological Sciences, Oklahoma State University, Stillwater, OK 74078, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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